Anti-Cyclin H/p34 antibody [EPR3929]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] (AB92376)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin H/p34 with Purified ab92376 at 1/50 dilution (2.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] (AB92376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Cyclin H/p34 with Purified ab92376 at 1/50 dilution (2.6 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

• Flow Cyt

Lab

Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] (AB92376)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling Cyclin H/p34 with Purified ab92376 at 1 : 20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] (AB92376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Cyclin H/p34 with Purified ab92376 at 1/50 dilution (2.6 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

• WB

Unknown

Western blot - Anti-Cyclin H/p34 antibody [EPR3929] (AB92376)

All lanes:

Western blot - Anti-Cyclin H/p34 antibody [EPR3929] (ab92376) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate

Lane 4:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 5:

Mouse kidney lysate

Lane 6:

Mouse spleen lysate

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

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