Rabbit Recombinant Monoclonal Cyclin H/p34 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples.
Images
![Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--flow-cytometry-img181099.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551)")
![Western blot - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--western-blot-img170016.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunocytochemistry-immunofluorescence-img164962.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166485.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165252.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (AB247551)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Alternative names
Cyclin-H, MO15-associated protein, p34, p37, CCNH
Recommended products
Rabbit Recombinant Monoclonal Cyclin H/p34 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3929
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247551 is the carrier-free version of Anti-Cyclin H/p34 antibody [EPR3929] ab92376.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--flow-cytometry-img181099.jpg "Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)")
Flow Cytometry - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)
This data was developed using ab247551, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling Cyclin H/p34 with Purified ab247551 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Western blot - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--western-blot-img170016.jpg "Western blot - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)")
Western blot - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)
All lanes: Western blot - Anti-Cyclin H/p34 antibody [EPR3929] (Anti-Cyclin H/p34 antibody [EPR3929] ab92376) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 4: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 5: Mouse kidney lysate
Lane 6: Mouse spleen lysate
Lane 7: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunocytochemistry-immunofluorescence-img164962.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)")
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)
This data was developed using Anti-Cyclin H/p34 antibody [EPR3929] ab92376, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin H/p34 with Purified Anti-Cyclin H/p34 antibody [EPR3929] ab92376 at 1/50 dilution (2.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166485.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)
This data was developed using ab247551, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Cyclin H/p34 with Purified ab247551 at 1/50 dilution (2.6 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551), expandable thumbnail](https://content.abcam.com/products/images/cyclin-h-p34-antibody-epr3929-bsa-and-azide-free-ab247551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165252.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin H/p34 antibody [EPR3929] - BSA and Azide free (ab247551)
This data was developed using ab247551, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Cyclin H/p34 with Purified ab247551 at 1/50 dilution (2.6 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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