AB73208

Anti-Cyclin H/p34 (phospho T315) antibody

Name: Anti-Cyclin H/p34 (phospho T315) antibody (ab73208) | Abcam
Brand: Abcam Limited
SKU: AB73208
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Cyclin H/p34 phospho T315 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Cyclin-H phospho T315.

View Alternative Names

Cyclin-H, MO15-associated protein, p34, p37, CCNH

1 Images

Unknown

Western blot - Anti-Cyclin H/p34 (phospho T315) antibody (AB73208)

All lanes:

Western blot - Anti-Cyclin H/p34 (phospho T315) antibody (ab73208) at 1/500 dilution

Lane 1:

HeLa cell extract at 30 µg

Lane 2:

HeLa cell extract at 30 µg with immunising phosphopeptide

Predicted band size: 38 kDa

Observed band size: 34 kDa,90 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Cyclin-H phospho T315. The exact immunogen used to generate this antibody is proprietary information.

P51946

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab73208 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

See full target information Cyclin-H phospho T315

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 3:378 PubMed32665601

2020

ATM-associated signalling triggers the unfolded protein response and cell death in response to stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Hotokezaka,Ikuo Katayama,Takashi Nakamura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin H/p34 (phospho T315) antibody

Western blot - Anti-Cyclin H/p34 (phospho T315) antibody (AB73208)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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