AB85854

Anti-Cyclin K antibody

Name: Anti-Cyclin K antibody (ab85854) | Abcam
Brand: Abcam Limited
SKU: AB85854
Price: 430 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Cyclin K antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CCNK aa 500 to C-terminus.

View Alternative Names

CPR4, CCNK, Cyclin-K

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody (AB85854)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody (AB85854)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human lung carcinoma sections labeling Cyclin K with ab85854 at a dilution of 1/5000 (0.2 μg/mL). Detection : DAB

Supplier Data

Immunoprecipitation - Anti-Cyclin K antibody (AB85854)

Detection of human Cyclin K by western blot of immunoprecipitates. Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies : Affinity purified rabbit anti-Cyclin K antibody ab85854 used for IP at 6 μg per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated Cyclin K, ab85854 was used at 0.04 μg/mL. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Cyclin K antibody (ab85854)

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-Cyclin K antibody (AB85854)

Detection : Chemiluminescence
Cells prepared using NETN lysis buffer

All lanes:

Western blot - Anti-Cyclin K antibody (ab85854) at 0.04 µg/mL

Lane 1:

NIH3T3 whole cell lysate at 50 µg

Lane 2:

CH27 whole cell lysate at 50 µg

Lane 3:

TCMK-1 whole cell lysate at 50 µg

Lane 4:

BW5147.3 whole cell lysate at 50 µg

Lane 5:

CT26 whole cell lysate at 50 µg

Predicted band size: 64 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Cyclin K antibody (AB85854)

Detection : Chemiluminescence

Cells prepared using NETN lysis buffer

All lanes:

Western blot - Anti-Cyclin K antibody (ab85854) at 0.04 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Predicted band size: 64 kDa

false

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human CCNK aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75909

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab85854 was affinity purified using an epitope specific to Cyclin K immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).

See full target information CCNK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 9: PubMed34696162

2021

HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Nur Firdaus Isa,Olivier Bensaude,Nadiah C Aziz,Shona Murphy

Cell reports 36:109514 PubMed34380030

2021

HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Chai,Sunan Li,Morgan K Collins,Rongrong Li,Iqbal Ahmad,Silas F Johnson,Dylan A Frabutt,Zhichang Yang,Xiaojing Shen,Liangliang Sun,Jian Hu,Judd F Hultquist,B Matija Peterlin,Yong-Hui Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin K antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody (AB85854)

Immunoprecipitation - Anti-Cyclin K antibody (AB85854)

Western blot - Anti-Cyclin K antibody (AB85854)

Western blot - Anti-Cyclin K antibody (AB85854)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

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