Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free

13 product images

• 

  Western blot - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  Cyclin K Western blot staining using rabbit Anti-Cyclin K antibody

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Low expression: WI-38 (PMID: 9632813), MEF (PMID: 22547058)

  The identity of the lower MW band at approximately 45 kDa (in lane 6) is unknown.

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

  All lanes: Western blot - Anti-Cyclin K antibody [EPR28514-24] (Anti-Cyclin K antibody [EPR28514-24] ab316203) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg with 5% NFDM/TBST

  Lane 2: HeLa transfected with siRNA specifically targeting Cyclin K whole cell lysate at 10 µg with 5% NFDM/TBST

  Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 4: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 5: WI-38 (human fetal lung fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 6: ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 7: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 75 kDa, 124 kDa

  Exposure time: 180s

• 

  Western blot - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  Cyclin K Western blot staining using rabbit Anti-Cyclin K antibody

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Low expression: heart (PMID: 25004108).

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

  All lanes: Western blot - Anti-Cyclin K antibody [EPR28514-24] (Anti-Cyclin K antibody [EPR28514-24] ab316203) at 1/1000 dilution

  Lane 1: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 2: Rat heart tissue lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 124 kDa

  Exposure time: 180s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse testis (PMID: 25004108). The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: weak staining on rat skeletal muscle. The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: weak staining on mouse skeletal muscle (PMID: 25004108). The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/200 (2.58 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: almost no staining on human skeletal muscle. The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat testis. The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse breast cancer. The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/200 (2.58 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human breast carcinoma. The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Western blot - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Low expression: skeletal muscle (PMID: 25004108), heart (PMID: 25004108).

  The identity of the bands between 37 kDa and 50 kDa (in lanes 2-3? are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Cyclin K antibody [EPR28514-24] (Anti-Cyclin K antibody [EPR28514-24] ab316203) at 1/1000 dilution

  Lane 1: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 2: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 3: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 75 kDa, 36 kDa

  Exposure time: 103s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Cyclin K with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/200 (2.58 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human testis (PMID: 9632813). The section was incubated with Anti-Cyclin K antibody [EPR28514-24] ab316203 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Western blot - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  In lanes 2-4, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

  The identity of the lower MW band at approximately 45 kDa (in lane 1) is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Cyclin K antibody [EPR28514-24] (Anti-Cyclin K antibody [EPR28514-24] ab316203) at 1/1000 dilution

  Lane 1: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 75 kDa, 36 kDa

  Exposure time: 103s

• 

  Immunoprecipitation - Anti-Cyclin K antibody [EPR28514-24] - BSA and Azide free (ab316204)

  This data was developed using Anti-Cyclin K antibody [EPR28514-24] ab316203, the same antibody clone in a different buffer formulation.

  Cyclin K was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cyclin K antibody [EPR28514-24] ab316203 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
  
  Lane 2: Anti-Cyclin K antibody [EPR28514-24] ab316203 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cyclin K antibody [EPR28514-24] ab316203 in HeLa whole cell lysate

  All lanes: Immunoprecipitation - Anti-Cyclin K antibody [EPR28514-24] (Anti-Cyclin K antibody [EPR28514-24] ab316203) at 1/30 dilution

  All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 180s