AB96133

Anti-Cyclin T2/CCNT2 antibody

Name: Anti-Cyclin T2/CCNT2 antibody (ab96133) | Abcam
Brand: Abcam Limited
SKU: AB96133
Price: 495 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Cyclin T2/CCNT2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus.

View Alternative Names

Cyclin-T2, CycT2, CCNT2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T2/CCNT2 antibody (AB96133)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T2/CCNT2 antibody (AB96133)

ab96133, at a 1/200 dilution, staining Cyclin T2/CCNT2 in paraformaldehyde fixed A431 cells by Immunofluorescence.
Lower image : merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (AB96133)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (AB96133)

ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded H1299 (human cell line) xenograft by Immunohistochemistry.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (AB96133)

ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded mouse brain tissue by Immunohistochemistry.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Unknown

Western blot - Anti-Cyclin T2/CCNT2 antibody (AB96133)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Cyclin T2/CCNT2 antibody (ab96133) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II) (PubMed : 15563843, PubMed : 9499409). The activity of this complex is regulated by binding with 7SK snRNA (PubMed : 11713533). Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (PubMed : 16331689). Involved in early embryo development (By similarity).. (Microbial infection) Promotes transcriptional activation of early and late herpes simplex virus 1/HHV-1 promoters.

See full target information CCNT2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 15:1013-1023 PubMed33817287

2020

Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Xing,Jinhai Ren,Xiaonan Guo,Shukai Qiao,Tian Tian

PloS one 11:e0152254 PubMed27011207

2016

P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig.

Applications

Unspecified application

Species

Unspecified reactive species

Reza K Oqani,Tao Lin,Jae Eun Lee,Ki Myung Choi,Hyun Young Shin,Dong Il Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin T2/CCNT2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T2/CCNT2 antibody (AB96133)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (AB96133)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (AB96133)

Western blot - Anti-Cyclin T2/CCNT2 antibody (AB96133)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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