Rabbit Polyclonal Cyclin T2/CCNT2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus.
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Publications
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- Open life sciences 15:1013-10232020Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Xing,Jinhai Ren,Xiaonan Guo,Shukai Qiao,Tian TianPubMed 33817287
- PloS one 11:e01522542016P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReza K Oqani et. al.PubMed 27011207
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60583
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II) (PubMed:15563843, PubMed:9499409). The activity of this complex is regulated by binding with 7SK snRNA (PubMed:11713533). Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (PubMed:16331689). Involved in early embryo development (By similarity). (Microbial infection) Promotes transcriptional activation of early and late herpes simplex virus 1/HHV-1 promoters.
Alternative names
Cyclin-T2, CycT2, CCNT2
Recommended products
Rabbit Polyclonal Cyclin T2/CCNT2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CCNT2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60583
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (ab96133)
ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded mouse brain tissue by Immunohistochemistry.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-Cyclin T2/CCNT2 antibody (ab96133)
7.5% SDS PAGE
All lanes: Western blot - Anti-Cyclin T2/CCNT2 antibody (ab96133) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 81 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T2/CCNT2 antibody (ab96133)
ab96133, at a 1/200 dilution, staining Cyclin T2/CCNT2 in paraformaldehyde fixed A431 cells by Immunofluorescence.
Lower image: merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2/CCNT2 antibody (ab96133)
ab96133, at a 1/100 dilution, staining Cyclin T2/CCNT2 in paraffin embedded H1299 (human cell line) xenograft by Immunohistochemistry.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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