Anti-Cyclophilin A antibody [EPR7511]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

ab126738 at 1/100 dilution staining Cyclophilin A in Formalin fixed Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 µg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the human kidney. The section was incubated with ab126738 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 µg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Low expression : weak staining on the human skeletal muscle. The section was incubated with ab126738 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 µg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the mouse kidney. The section was incubated with ab126738 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 µg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Low expression : weak staining on the mouse cardiac muscle. The section was incubated with ab126738 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST Low expression control : mouse heart. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

All lanes:

Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738) at 1/1000 dilution

Lane 1:

K562 lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 18 kDa

true

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Cyclophilin A antibody [EPR7511] (AB126738)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.