Anti-Cyclophilin B antibody [CL3901] - Loading Control

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

• WB

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Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Lanes 1 - 4 : Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab236760 was shown to specifically react with PPIB in wild-type HAP1 cells as signal was lost in PPIB knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab236760 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PPIB knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Jurkat whole cell lysate at 20 µg

Lane 4:

U87-MG whole cell lysate at 20 µg

Predicted band size: 24 kDa

false

• WB

Lab

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

Lanes 1-4 : Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

ab236760 Anti-Cyclophilin B antibody [CL3901] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261746 (knockout cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab236760 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ppib-cyclophilin-b-knockout-hela-cell-line-ab261746'>ab261746</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760)

All lanes:

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL

Lane 1:

Control siRNA transfected U-251 MG (human brain glioma cell line) cell lysate

Lane 2:

Cyclophilin B siRNA probe 1 transfected U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Cyclophilin B siRNA probe 2 transfected U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 24 kDa

false