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Mouse Monoclonal Cyclophilin B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPIB aa 150 to C-terminus.

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Images

Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760), expandable thumbnail
  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760), expandable thumbnail
  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760), expandable thumbnail
  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (AB236760), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human PPIB aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23284

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Chicken
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Chicken, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000.00000 - 1/10000.00000
Notes

HIER pH 6

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chicken, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.

Alternative names

Recommended products

Mouse Monoclonal Cyclophilin B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPIB aa 150 to C-terminus.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human PPIB aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23284
Clone number
CL3901
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

    Lanes 1-4: Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab236760 Anti-Cyclophilin B antibody [CL3901] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746 (knockout cell lysate Human PPIB (Cyclophilin B) knockout HeLa cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab236760 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/1000 dilution

    Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746)

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa

    Observed band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

    Lanes 1 - 4: Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab236760 was shown to specifically react with PPIB in wild-type HAP1 cells as signal was lost in PPIB knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab236760 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/5000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: PPIB knockout HAP1 whole cell lysate at 20 µg

    Lane 3: Jurkat whole cell lysate at 20 µg

    Lane 4: U87-MG whole cell lysate at 20 µg

    Predicted band size: 24 kDa

  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

    All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL

    Lane 1: Control siRNA transfected U-251 MG (human brain glioma cell line) cell lysate

    Lane 2: Cyclophilin B siRNA probe 1 transfected U-251 MG (human brain glioma cell line) cell lysate

    Lane 3: Cyclophilin B siRNA probe 2 transfected U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 24 kDa

  • Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

    All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lane 3: A431 (human epidermoid carcinoma cell line) cell lysate

    Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lane 6: NBT II (rat cell line) cell lysate

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

    Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

    Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

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Product protocols

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