Mouse Monoclonal Cyclophilin B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPIB aa 150 to C-terminus.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Expected |
Chicken | Predicted | Predicted |
Cow | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/10000.00000 | Notes HIER pH 6 |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
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PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
CYPB, PPIB, Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP
Mouse Monoclonal Cyclophilin B antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPIB aa 150 to C-terminus.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody
ab236760 Anti-Cyclophilin B antibody [CL3901] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746 (knockout cell lysate Human PPIB (Cyclophilin B) knockout HeLa cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab236760 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/1000 dilution
Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746)
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody
Formalin-fixed, paraffin-embedded human stomach tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.
Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody
ab236760 was shown to specifically react with PPIB in wild-type HAP1 cells as signal was lost in PPIB knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab236760 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/5000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: PPIB knockout HAP1 whole cell lysate at 20 µg
Lane 3: Jurkat whole cell lysate at 20 µg
Lane 4: U87-MG whole cell lysate at 20 µg
Predicted band size: 24 kDa
Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody
All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL
Lane 1: Control siRNA transfected U-251 MG (human brain glioma cell line) cell lysate
Lane 2: Cyclophilin B siRNA probe 1 transfected U-251 MG (human brain glioma cell line) cell lysate
Lane 3: Cyclophilin B siRNA probe 2 transfected U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 24 kDa
Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody
All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3: A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 6: NBT II (rat cell line) cell lysate
Predicted band size: 24 kDa
Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody
Formalin-fixed, paraffin-embedded human placenta tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.
Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody
Formalin-fixed, paraffin-embedded human prostate tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.
Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.
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