Anti-Cyclophilin B antibody [CL3901] - Loading Control

8 product images

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  Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

  Lanes 1-4: Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
  

  ab236760 Anti-Cyclophilin B antibody [CL3901] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746 (knockout cell lysate Human PPIB (Cyclophilin B) knockout HeLa cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab236760 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/1000 dilution

  Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

  Lane 2: PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

  Lane 2: Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746)

  Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

  Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

  Formalin-fixed, paraffin-embedded human stomach tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

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  Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

  Lanes 1 - 4: Merged signal (red and green). Green - ab236760 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
  

  ab236760 was shown to specifically react with PPIB in wild-type HAP1 cells as signal was lost in PPIB knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab236760 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1/5000 dilution

  Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

  Lane 2: PPIB knockout HAP1 whole cell lysate at 20 µg

  Lane 3: Jurkat whole cell lysate at 20 µg

  Lane 4: U87-MG whole cell lysate at 20 µg

  Predicted band size: 24 kDa

• 

  Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

  All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL

  Lane 1: Control siRNA transfected U-251 MG (human brain glioma cell line) cell lysate

  Lane 2: Cyclophilin B siRNA probe 1 transfected U-251 MG (human brain glioma cell line) cell lysate

  Lane 3: Cyclophilin B siRNA probe 2 transfected U-251 MG (human brain glioma cell line) cell lysate

  Predicted band size: 24 kDa

• 

  Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Western blot staining using mouse Anti-Cyclophilin B antibody

  All lanes: Western blot - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760) at 1 µg/mL

  Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

  Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

  Lane 3: A431 (human epidermoid carcinoma cell line) cell lysate

  Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

  Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

  Lane 6: NBT II (rat cell line) cell lysate

  Predicted band size: 24 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

  Formalin-fixed, paraffin-embedded human placenta tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

  Formalin-fixed, paraffin-embedded human prostate tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin B antibody [CL3901] - Loading Control (ab236760)

  Cyclophilin B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-Cyclophilin B antibody

  Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Cyclophilin B with ab236760 at a 1/5000 dilution in immunohistochemical analysis.