Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178397).

Lanes 1 - 4 : Merged signal (red and green). Green - ab178397 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab178397 was shown to recognize PPIB in wild-type HAP1 cells as signal was lost at the expected MW in PPIB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab178397 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991) at 1 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg/mL

Lane 2:

PPIB knockout HAP1 whole cell lysate at 20 µg/mL

Lane 3:

Jurkat whole cell lysate at 20 µg/mL

Lane 4:

U87-MG whole cell lysate at 20 µg/mL

Predicted band size: 24 kDa

false

• WB

Lab

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)

This data was developed using ab178397, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab178397 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

ab178397 Anti-Cyclophilin B antibody [EPR12703(B)] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261746 (knockout cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. ab178397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (<a href='/en-us/products/primary-antibodies/cyclophilin-b-antibody-epr12703b-loading-control-ab178397'>ab178397</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ppib-cyclophilin-b-knockout-hela-cell-line-ab261746'>ab261746</a>)

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab178397).

All lanes:

Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (<a href='/en-us/products/primary-antibodies/cyclophilin-b-antibody-epr12703b-loading-control-ab178397'>ab178397</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 24 kDa

true