Rabbit Recombinant Monoclonal Cyclophilin B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img4989.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)")
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img138373.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)")
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img106891.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (AB238991)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Expected | Not recommended | Not recommended |
| Rat | Not recommended | Expected | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
1 product for Alternative Version
Target data
Function
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
Alternative names
CYPB, PPIB, Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP
Recommended products
Rabbit Recombinant Monoclonal Cyclophilin B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12703(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238991 is the carrier-free version of Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img4989.jpg "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)")
Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
This data was developed using Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 Anti-Cyclophilin B antibody [EPR12703(B)] was shown to specifically react with Cyclophilin B in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746 (knockout cell lysate Human PPIB (Cyclophilin B) knockout HeLa cell lysate ab257037) was used. Wild-type and Cyclophilin B knockout samples were subjected to SDS-PAGE. Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397) at 1/1000 dilution
Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: PPIB knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell line (Human PPIB (Cyclophilin B) knockout HeLa cell line ab261746)
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img138373.jpg "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)")
Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 was shown to recognize PPIB in wild-type HAP1 cells as signal was lost at the expected MW in PPIB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PPIB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991) at 1 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg/mL
Lane 2: PPIB knockout HAP1 whole cell lysate at 20 µg/mL
Lane 3: Jurkat whole cell lysate at 20 µg/mL
Lane 4: U87-MG whole cell lysate at 20 µg/mL
Predicted band size: 24 kDa
![Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991), expandable thumbnail](https://content.abcam.com/products/images/cyclophilin-b-antibody-epr12703b-bsa-and-azide-free-ab238991--western-blot-img106891.jpg "Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)")
Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397).
All lanes: Western blot - Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control ab178397) at 1/1000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
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