JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253289

Anti-Cyclophilin B antibody [M019] - Loading Control

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal Cyclophilin B antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPIB.

View Alternative Names

CYPB, PPIB, Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody [M019] - Loading Control (AB253289)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody [M019] - Loading Control (AB253289)

Methanol/Acetone (1 : 1) fixed, A549 (Human lung carcinoma cell line) cells labeling Cyclophilin B using ab253289 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

Western blot - Anti-Cyclophilin B antibody [M019] - Loading Control (AB253289)
  • WB

Supplier Data

Western blot - Anti-Cyclophilin B antibody [M019] - Loading Control (AB253289)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.

All lanes:

Western blot - Anti-Cyclophilin B antibody [M019] - Loading Control (ab253289) at 1/1000 dilution

Lane 1:

LNCaP (Human prostate cancer cell line) cell lysate

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) cell lysate

Lane 3:

MDA-MB-231 (Human cell line) cell lysate

Lane 4:

MeWo (Human malignant melanoma cell line) cell lysate

Lane 5:

Recombinant full length Cyclophilin B protein

Lane 6:

Recombinant full length Cyclophilin A protein

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M019

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PPIB. The exact immunogen used to generate this antibody is proprietary information.

P23284

Specificity

Detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
See full target information PPIB

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 18:172 PubMed34372877

2021

Increasing toll-like receptor 2 on astrocytes induced by Schwann cell-derived exosomes promotes recovery by inhibiting CSPGs deposition after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dayu Pan,Yongjin Li,Fuhan Yang,Zenghui Lv,Shibo Zhu,Yixin Shao,Ying Huang,Guangzhi Ning,Shiqing Feng
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com