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Mouse Monoclonal Cyclophilin F antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 112 publications.
Alternative names=PPIase F, Cyclophilin D, Cyclophilin F, Mitochondrial cyclophilin, Rotamase F, CyP-D, CypD, CyP-M, PPIF, CYP3
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Expected | Expected | Tested | Expected |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Recommended products
Mouse Monoclonal Cyclophilin F antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow samples. Cited in 112 publications.
Alternative names=PPIase F, Cyclophilin D, Cyclophilin F, Mitochondrial cyclophilin, Rotamase F, CyP-D, CypD, CyP-M, PPIF, CYP3
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
E11AE12BD4
- Light chain type
kappa
- Concentration
- Purification notes
ab110324 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
This monoclonal antibody to cyclophilin F has been knockout validated in Western blot. The expected band for cyclophilin F was observed in wild type cells and the band was not seen in knockout cells.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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