AB108220

Anti-CYFIP1 antibody

Name: Anti-CYFIP1 antibody (ab108220) | Abcam
Brand: Abcam Limited
SKU: AB108220
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CYFIP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYFIP1 aa 1200 to C-terminus.

View Alternative Names

KIAA0068, CYFIP1, Cytoplasmic FMR1-interacting protein 1, Specifically Rac1-associated protein 1, p140sra-1, Sra-1

2 Images

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Western blot - Anti-CYFIP1 antibody (AB108220)

All lanes:

Western blot - Anti-CYFIP1 antibody (ab108220) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 145 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-CYFIP1 antibody (AB108220)

ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes an upstream epitope of CYFIP1
Lane 2 : ab108220 at 6 µg/mg of lysate
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-CYFIP1 antibody (ab108220)

Predicted band size: 145 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CYFIP1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L576

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Lizard": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYFIP1 also known as cytoplasmic FMR1-interacting protein 1 plays an important role in cellular processes including actin dynamics and translation regulation. This protein which has a mass of approximately 140 kDa interacts with FMRP and links to the ribosome controlling mRNA translation. CYFIP1 is expressed mostly in the brain but is also present in other tissues like blood. Researchers study CYFIP1 to understand its role in neural activity and cellular signaling processes.

Biological function summary

CYFIP1 operates as part of the WAVE regulatory complex which is critical for actin polymerization. This complex facilitates cytoskeletal reorganization essential for cellular movement and morphology. CYFIP1 influences synaptic plasticity an important process in neural signaling and cognitive functions. By interacting with other molecular components CYFIP1 impacts processes like memory formation and learning.

Pathways

CYFIP1 is integral to the Rac1 and Arp2/3 pathways which directly affect actin filament assembly. In these pathways CYFIP1 interacts with proteins such as NCKAP1 and ABI2 which play roles in the regulation of actin cytoskeleton reorganization. The involvement of CYFIP1 in these pathways highlights its importance in processes that require rapid cytoskeletal changes such as cell migration and synaptic growth.

CYFIP1's function draws a connection to neurodevelopmental disorders and schizophrenia. Alterations in the expression of CYFIP1 have been observed in these conditions suggesting a link between its dysregulation and abnormal brain development. Moreover its interaction with the FMRP protein ties it to Fragile X syndrome characterized by cognitive impairment. Research into these associations provides valuable insight into potential therapeutic targets for these complex conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). May act as an invasion suppressor in cancers.

See full target information CYFIP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neuron 97:1078-1093.e6 PubMed29518358

2018

Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Michel Cioni,Hovy Ho-Wai Wong,Dario Bressan,Lay Kodama,William A Harris,Christine E Holt

Human molecular genetics 24:1813-23 PubMed25432536

2014

Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kihoon Han,Hogmei Chen,Vincenzo A Gennarino,Ronald Richman,Hui-Chen Lu,Huda Y Zoghbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYFIP1 antibody

Western blot - Anti-CYFIP1 antibody (AB108220)

Immunoprecipitation - Anti-CYFIP1 antibody (AB108220)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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