Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (AB249255)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/50 dilution (5.56 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (AB249255)

Intracellular Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/30 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

• WB

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Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (AB249255)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

All lanes:

Western blot - Anti-CYFIP1 antibody [EPR10782] (<a href='/en-us/products/primary-antibodies/cyfip1-antibody-epr10782-ab156016'>ab156016</a>) at 1/1000 dilution

Lane 1:

Human brain lysates at 20 µg

Lane 2:

Human lung lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Mouse lung lysates at 20 µg

Lane 5:

Mouse breast lysates at 20 µg

Lane 6:

Rat brain lysates at 20 µg

Lane 7:

Rat lung lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 145 kDa

Observed band size: 145 kDa

false

• WB

Unknown

Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (AB249255)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

All lanes:

Western blot - Anti-CYFIP1 antibody [EPR10782] (<a href='/en-us/products/primary-antibodies/cyfip1-antibody-epr10782-ab156016'>ab156016</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 3:

A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 6:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 9:

Rat-1 (Rat embryonic fibroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 145 kDa

Observed band size: 145 kDa

false