AB95969

Anti-CYFIP2 antibody

Name: Anti-CYFIP2 antibody (ab95969) | Abcam
Brand: Abcam Limited
SKU: AB95969
Rating: 3 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal CYFIP2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYFIP2 aa 300-650.

View Alternative Names

KIAA1168, PIR121, CYFIP2, Cytoplasmic FMR1-interacting protein 2, p53-inducible protein 121

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 antibody (AB95969)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 antibody (AB95969)

Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue labelling CYFIP2 protein at cytoplasm and nucleus with ab95969 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CYFIP2 aa 300-650. The exact immunogen used to generate this antibody is proprietary information.

Q96F07

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'CYFIP2' protein also known as the Cytoplasmic FMR1 Interacting Protein 2 plays a critical role in cellular processes. It has a molecular mass of approximately 140 kDa. CYFIP2 is widely expressed in various tissues but its expression is particularly notable in the brain. It acts as a component of a protein complex that regulates the actin cytoskeleton which is essential for maintaining cell structure and function.

Biological function summary

CYFIP2 influences cellular signaling and synaptic growth. It forms part of the WAVE regulatory complex playing a part in actin filament assembly by nucleating actin-related proteins. This biological activity is essential for neurite outgrowth and synaptic plasticity processes important for proper neural function. CYFIP2's interaction with other components of the neuronal cytoskeleton reveals its importance in nervous system development.

Pathways

CYFIP2 integrates into the Rho-GTPase signaling and mTOR pathways. It acts in conjunction with other proteins like Rac1 which activates the WAVE complex leading to actin cytoskeleton remodeling. This coordination is critical for cellular functions such as morphogenesis migration and intracellular transport. Another important interaction involves mTOR signaling as CYFIP2 influences protein synthesis and cell growth through this pathway.

Dysfunction in CYFIP2 links to neurological conditions including intellectual disability and autism spectrum disorders. These disorders arise due to mutations affecting CYFIP2's role in synaptic plasticity and development. CYFIP2's interaction with FMRP (Fragile X Mental Retardation Protein) plays a significant role as both proteins coordinate to manage synaptic signaling and structural stability in neural tissues with anomalies potentially leading to disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in T-cell adhesion and p53/TP53-dependent induction of apoptosis. Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).

See full target information CYFIP2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Animal cells and systems 28:294-302 PubMed38832126

2024

Cell-autonomous reduction of CYFIP2 changes dendrite length, dendritic protrusion morphology, and inhibitory synapse density in the hippocampal CA1 pyramidal neurons of 17-month-old mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yoonhee Kim,Ruiying Ma,Yinhua Zhang,Hyae Rim Kang,U Suk Kim,Kihoon Han

Animal cells and systems 27:93-101 PubMed36999135

2023

Cell-autonomous reduction of CYFIP2 is insufficient to induce Alzheimer's disease-like pathologies in the hippocampal CA1 pyramidal neurons of aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiying Ma,Yinhua Zhang,Huiling Li,Hyae Rim Kang,Yoonhee Kim,Kihoon Han

Proceedings of the National Academy of Sciences of the United States of America 120:e2215011120 PubMed36917665

2023

The WAVE complex drives the morphogenesis of the photoreceptor outer segment cilium.

Applications

Unspecified application

Species

Unspecified reactive species

William J Spencer,Nicholas F Schneider,Tylor R Lewis,Carson M Castillo,Nikolai P Skiba,Vadim Y Arshavsky

Annals of neurology 93:155-163 PubMed36251395

2022

CYFIP2 p.Arg87Cys Causes Neurological Defects and Degradation of CYFIP2.

Applications

Unspecified application

Species

Unspecified reactive species

Muwon Kang,Yinhua Zhang,Hyae Rim Kang,Seoyeong Kim,Ruiying Ma,Yunho Yi,Seungjoon Lee,Yoonhee Kim,Huiling Li,Chunmei Jin,Dongmin Lee,Eunjoon Kim,Kihoon Han

Frontiers in molecular neuroscience 13:574947 PubMed33192297

2020

Enhanced Prefrontal Neuronal Activity and Social Dominance Behavior in Postnatal Forebrain Excitatory Neuron-Specific Knock-Out Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yinhua Zhang,Rim Kang Hyae,Seung-Hyun Lee,Yoonhee Kim,Ruiying Ma,Chunmei Jin,Ji-Eun Lim,Seoyeon Kim,Yeju Kang,Hyojin Kang,Su Yeon Kim,Seok-Kyu Kwon,Se-Young Choi,Kihoon Han

Function (Oxford, England) 1:zqaa013 PubMed34278304

2020

Adipose Tissue Inflammation Is Directly Linked to Obesity-Induced Insulin Resistance, while Gut Dysbiosis and Mitochondrial Dysfunction Are Not Required.

Applications

Species

Unspecified reactive species

Heather L Petrick,Kevin P Foley,Soumaya Zlitni,Henver S Brunetta,Sabina Paglialunga,Paula M Miotto,Valerie Politis-Barber,Conor O'Dwyer,Diana J Philbrick,Morgan D Fullerton,Jonathan D Schertzer,Graham P Holloway

Annals of neurology 88:526-543 PubMed32562430

2020

Haploinsufficiency of Cyfip2 Causes Lithium-Responsive Prefrontal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Hyun Lee,Yinhua Zhang,Jina Park,Bowon Kim,Yangsik Kim,Sang Hoon Lee,Gyu Hyun Kim,Yang Hoon Huh,Bokyoung Lee,Yoonhee Kim,Yeunkum Lee,Jin Yong Kim,Hyojin Kang,Su-Yeon Choi,Seil Jang,Yan Li,Shinhyun Kim,Chunmei Jin,Kaifang Pang,Eunjeong Kim,Yoontae Lee,Hyun Kim,Eunjoon Kim,Jee Hyun Choi,Jeongjin Kim,Kea Joo Lee,Se-Young Choi,Kihoon Han

Animal cells and systems 23:380-383 PubMed31853374

2019

Differential cell-type-expression of CYFIP1 and CYFIP2 in the adult mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Yinhua Zhang,Hyae Rim Kang,Kihoon Han

Cell reports 23:878-887 PubMed29669291

2018

A Cyfip2-Dependent Excitatory Interneuron Pathway Establishes the Innate Startle Threshold.

Applications

IHC

Species

Zebrafish

Kurt C Marsden,Roshan A Jain,Marc A Wolman,Fabio A Echeverry,Jessica C Nelson,Katharina E Hayer,Ben Miltenberg,Alberto E Pereda,Michael Granato

Human molecular genetics 24:1813-23 PubMed25432536

2014

Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kihoon Han,Hogmei Chen,Vincenzo A Gennarino,Ronald Richman,Hui-Chen Lu,Huda Y Zoghbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYFIP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 antibody (AB95969)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise