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Rabbit Recombinant Monoclonal CYFIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.

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Images

Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (AB204129), expandable thumbnail
  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (AB204129), expandable thumbnail
  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (AB204129), expandable thumbnail
  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (AB204129), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (AB204129), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). May act as an invasion suppressor in cancers.

Additional Targets

CYFIP2

Alternative names

KIAA0068, CYFIP1, Cytoplasmic FMR1-interacting protein 1, Specifically Rac1-associated protein 1, p140sra-1, Sra-1

Recommended products

Rabbit Recombinant Monoclonal CYFIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17848-87
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The proteins CYFIP2 and CYFIP1 also known as cytoplasmic FMR1-interacting proteins 2 and 1 are mechanistically involved in actin cytoskeleton regulation. Their molecular weights are approximately 147 kDa for CYFIP2 and 140 kDa for CYFIP1. Both proteins are widely expressed across various tissues with notable expression in the brain and other neural tissues. CYFIP2 and CYFIP1 interact with the WAVE regulatory complex influencing actin dynamics and in turn cellular morphology and movement.

Biological function summary

These proteins function by serving as scaffolds in cellular processes impacting synaptic plasticity and neuronal function. CYFIP1 and CYFIP2 are integral members of the WAVE regulatory complex which is important for inducing actin polymerization. By associating with FMRP (Fragile X Mental Retardation Protein) CYFIP proteins help modulate mRNA translation linked to synaptic development. This involvement in synaptic modulation highlights their role in the nervous system's regulatory mechanisms.

Pathways

Both CYFIP1 and CYFIP2 play key roles in the RAC1/CDC42 signaling pathway critical for actin filament organization. They interact with proteins such as WAVE Arp2/3 complex and FMRP linking them to processes that control cell shape and motility. This connection to the RAC1/CDC42 pathway and their association with the FMRP complex highlight their involvement in cytoskeleton reorganization and mRNA transport.

Associated diseases and disorders

CYFIP2 and CYFIP1 have associations with neurological conditions like Fragile X syndrome and autism spectrum disorders. These proteins through their interaction with FMRP influence synaptic connectivity and function indicating their potential role in these diseases. Additionally CYFIP1 dysregulation has been linked to schizophrenia possibly through disruptions in actin cytoskeleton signaling and related synaptic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/50000 dilution

    All lanes: Human CYFIP1 Recombinant protein fragment at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa

    Observed band size: 148 kDa

    Exposure time: 10s

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 2: Molt4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human fetal brain lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa

    Observed band size: 148 kDa

    Exposure time: 1s

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa

    Observed band size: 148 kDa

    Exposure time: 15s

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/1000 dilution

    All lanes: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa

    Observed band size: 146 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CYFIP2 + CYFIP1 with ab204129 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on human tonsil tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on JurKat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized SW480 (Human colon adenocarcinoma cell line) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

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Product protocols

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