Goat Polyclonal CYP1A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cyp1a1 aa 250-350.
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- BMC pharmacology & toxicology 23:82022Sex-dependent effects of long-term clozapine or haloperidol medication on red blood cells and liver iron metabolism in Sprague Dawley rats as a model of metabolic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Luise Bouvier,Karin Fehsel,Andrea Schmitt,Eva Meisenzahl-Lechner,Wolfgang Gaebel,Martina von WilmsdorffPubMed 35033194
- International journal of molecular medicine 48:2021Taxifolin suppresses the malignant progression of gastric cancer by regulating the AhR/CYP1A1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiebin Xie,Yueshan Pang,Xiaoting WuPubMed 34490474
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Cyp1a1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P00184
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Expected | Expected | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).
Alternative names
Cytochrome P450 1A1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, Hydroperoxy icosatetraenoate dehydratase, CYP1A1
Recommended products
Goat Polyclonal CYP1A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cyp1a1 aa 250-350.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Cyp1a1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link P00184
- Purification technique
- Affinity purification Immunogen
- Specificity
ab126887 is not expected to cross-react with the similar P450 1A2.
- Concentration
- Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP1A1 also known as cytochrome P450 1A1 is an enzyme that plays a critical role in the metabolism of xenobiotics. CYP1A1 facilitates the oxidation of polycyclic aromatic hydrocarbons (PAHs) and other environmental toxins converting them into more soluble compounds. With a molecular mass of approximately 58 kDa CYP1A1 locates primarily in the endoplasmic reticulum. Expression of CYP1A1 takes place mainly in the liver although it also appears in the lungs and intestines.
- Biological function summary
Cytochrome P450 1A1 acts in detoxification processes and is involved in bioactivation of precarcinogens. The enzyme is part of the cytochrome P450 family which consists of many enzymes responsible for metabolizing diverse substrates. CYP1A1's activity can lead to the activation of carcinogens if bioactivated compounds form DNA adducts potentially leading to mutagenesis.
- Pathways
CYP1A1 interacts in the aryl hydrocarbon receptor (AhR) signaling pathway and xenobiotic metabolism pathway. In the AhR pathway CYP1A1 participates in the induction of metabolism of environmental pollutants. Within these pathways it works alongside other cytochrome P450 enzymes like CYP1A2 and CYP1B1 both important in similar detoxification processes. The enzyme's regulation affects chemical homeostasis impacting the removal of potential toxins from the body.
- Associated diseases and disorders
CYP1A1's activity connects closely with lung cancer and cardiovascular diseases. This enzyme upon metabolizing PAHs and similar substances results in metabolites that contribute to carcinogenesis affecting lung tissue. Likewise by-products of its activity link to vascular inflammatory processes influencing cardiovascular health. Interactions with proteins such as AhR and Nrf2 modulate responses that can mitigate or exacerbate these conditions depending on the detoxification efficacy and the balance between bioactivation and elimination of harmful compounds.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-CYP1A1 antibody (ab126887)
All lanes: Western blot - Anti-CYP1A1 antibody (ab126887) at 0.1 µg/mL
All lanes: Rat lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (ab126887)
Immunohistochemistry analysis of paraffin embedded human tonsil tissue labeling CYP1A1 with ab126887. Heat induced antigen retrieval was performed with Tris-EDTA pH 9, HRP-Staining (1/500) (left).Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody (right).
Western blot - Anti-CYP1A1 antibody (ab126887)
Detected by chemiluminescence.
All lanes: Western blot - Anti-CYP1A1 antibody (ab126887) at 0.1 µg/mL
All lanes: Human Lung lysate (35 µg protein in RIPA buffer)
Predicted band size: 58 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (ab126887)
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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