Rabbit Polyclonal CYP1A1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Images
Publications
Filter by
- The FEBS journal 290:837-8542022Fusobacterium nucleatum promotes proliferation in oesophageal squamous cell carcinoma via AHR/CYP1A1 signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaisen Yin,Jianwei Zhang,Hanping Zhang,Qing Li,Hu Qiu,Kunqiao Hong,Wei Wang,Yong Xiao,Baoping YuPubMed 36070107
- Frontiers in nutrition 9:8822222022Combinatory Exposure to Urolithin A, Alternariol, and Deoxynivalenol Affects Colon Cancer Metabolism and Epithelial Barrier Integrity .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Groestlinger,Carina Seidl,Elisabeth Varga,Giorgia Del Favero,Doris MarkoPubMed 35811943
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Incubate with primary antibody for 30 minutes at RT. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 15 µg/mL | Notes Incubate with primary antibody overnight at 4°C. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).
Alternative names
Cytochrome P450 1A1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, Hydroperoxy icosatetraenoate dehydratase, CYP1A1
Recommended products
Rabbit Polyclonal CYP1A1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP1A1 also known as cytochrome P450 1A1 is an enzyme that plays a critical role in the metabolism of xenobiotics. CYP1A1 facilitates the oxidation of polycyclic aromatic hydrocarbons (PAHs) and other environmental toxins converting them into more soluble compounds. With a molecular mass of approximately 58 kDa CYP1A1 locates primarily in the endoplasmic reticulum. Expression of CYP1A1 takes place mainly in the liver although it also appears in the lungs and intestines.
- Biological function summary
Cytochrome P450 1A1 acts in detoxification processes and is involved in bioactivation of precarcinogens. The enzyme is part of the cytochrome P450 family which consists of many enzymes responsible for metabolizing diverse substrates. CYP1A1's activity can lead to the activation of carcinogens if bioactivated compounds form DNA adducts potentially leading to mutagenesis.
- Pathways
CYP1A1 interacts in the aryl hydrocarbon receptor (AhR) signaling pathway and xenobiotic metabolism pathway. In the AhR pathway CYP1A1 participates in the induction of metabolism of environmental pollutants. Within these pathways it works alongside other cytochrome P450 enzymes like CYP1A2 and CYP1B1 both important in similar detoxification processes. The enzyme's regulation affects chemical homeostasis impacting the removal of potential toxins from the body.
- Associated diseases and disorders
CYP1A1's activity connects closely with lung cancer and cardiovascular diseases. This enzyme upon metabolizing PAHs and similar substances results in metabolites that contribute to carcinogenesis affecting lung tissue. Likewise by-products of its activity link to vascular inflammatory processes influencing cardiovascular health. Interactions with proteins such as AhR and Nrf2 modulate responses that can mitigate or exacerbate these conditions depending on the detoxification efficacy and the balance between bioactivation and elimination of harmful compounds.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-CYP1A1 antibody (ab235185)
Blocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.All lanes: Western blot - Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution
Lane 1: CYP1A1 overexpressing lysate
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell line) lysate
SecondaryAll lanes: Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution
Predicted band size: 58 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (ab235185)
Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.
Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.
Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.
A. Human kidney tissue counterstained with Hematoxilyn-Eosin.
B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).
C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).
Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (ab235185)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 μg/ml in ICC/IF.
Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 μg/ml for 1 hour at room temperature.
Panel A: DAPI (blue)
Panel B: ab235185 + secondary antibody (green)
Panel C: Actin-Phalloidin 555 (red)
Panel D: Secondary antibody only
Panel E: Merge A+B
Panel F: Merge A+B+C
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com