AB235185

Anti-CYP1A1 antibody

Name: Anti-CYP1A1 antibody (ab235185) | Abcam
Brand: Abcam Limited
SKU: AB235185
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal CYP1A1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Cytochrome P450 1A1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, Hydroperoxy icosatetraenoate dehydratase, CYP1A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (AB235185)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (AB235185)

Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.

Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.

Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.

A. Human kidney tissue counterstained with Hematoxilyn-Eosin.

B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).

C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).

Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (AB235185)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (AB235185)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 μg/ml in ICC/IF.

Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 μg/ml for 1 hour at room temperature.

Panel A : DAPI (blue)

Panel B : ab235185 + secondary antibody (green)

Panel C : Actin-Phalloidin 555 (red)

Panel D : Secondary antibody only

Panel E : Merge A+B

Panel F : Merge A+B+C

Supplier Data

Western blot - Anti-CYP1A1 antibody (AB235185)

Blocking/Dilution buffer : Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.

All lanes:

Western blot - Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution

Lane 1:

CYP1A1 overexpressing lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell line) lysate

Secondary

All lanes:

Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution

Predicted band size: 58 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Incubate with primary antibody for 30 minutes at RT. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "Incubate with primary antibody overnight at 4°C." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP1A1 also known as cytochrome P450 1A1 is an enzyme that plays a critical role in the metabolism of xenobiotics. CYP1A1 facilitates the oxidation of polycyclic aromatic hydrocarbons (PAHs) and other environmental toxins converting them into more soluble compounds. With a molecular mass of approximately 58 kDa CYP1A1 locates primarily in the endoplasmic reticulum. Expression of CYP1A1 takes place mainly in the liver although it also appears in the lungs and intestines.

Biological function summary

Cytochrome P450 1A1 acts in detoxification processes and is involved in bioactivation of precarcinogens. The enzyme is part of the cytochrome P450 family which consists of many enzymes responsible for metabolizing diverse substrates. CYP1A1's activity can lead to the activation of carcinogens if bioactivated compounds form DNA adducts potentially leading to mutagenesis.

Pathways

CYP1A1 interacts in the aryl hydrocarbon receptor (AhR) signaling pathway and xenobiotic metabolism pathway. In the AhR pathway CYP1A1 participates in the induction of metabolism of environmental pollutants. Within these pathways it works alongside other cytochrome P450 enzymes like CYP1A2 and CYP1B1 both important in similar detoxification processes. The enzyme's regulation affects chemical homeostasis impacting the removal of potential toxins from the body.

CYP1A1's activity connects closely with lung cancer and cardiovascular diseases. This enzyme upon metabolizing PAHs and similar substances results in metabolites that contribute to carcinogenesis affecting lung tissue. Likewise by-products of its activity link to vascular inflammatory processes influencing cardiovascular health. Interactions with proteins such as AhR and Nrf2 modulate responses that can mitigate or exacerbate these conditions depending on the detoxification efficacy and the balance between bioactivation and elimination of harmful compounds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed : 10681376, PubMed : 11555828, PubMed : 12865317, PubMed : 14559847, PubMed : 15041462, PubMed : 15805301, PubMed : 18577768, PubMed : 19965576, PubMed : 20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 10681376, PubMed : 11555828, PubMed : 12865317, PubMed : 14559847, PubMed : 15041462, PubMed : 15805301, PubMed : 18577768, PubMed : 19965576, PubMed : 20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed : 11555828, PubMed : 12865317, PubMed : 14559847, PubMed : 15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed : 15041462, PubMed : 18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed : 15041462, PubMed : 19965576, PubMed : 20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed : 20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed : 15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed : 10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed : 21068195).

See full target information Cytochrome P450 1A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Balkan medical journal 42:440-451 PubMed40888349

2025

Inhibition of PI3K and Hedgehog Signaling Pathway Inhibits Hypoxia-Induced Vasculogenic Mimicry Formation in Ovarian Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liang,Yun Bai,Huan Zhao

General physiology and biophysics 44:303-315 PubMed40567073

2025

Protocatechuic acid alleviates chronic obstructive pulmonary disease model by reducing inflammation and oxidative stress via the AHR/CYP1A1/CYP1A2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Pan,Xiaowei Zhang,Ruizhi Li,Sheng Zhang,Chengda Zhang,Yongjia Zhu

Cellular & molecular biology letters 30:40 PubMed40175891

2025

A novel mechanism for A-to-I RNA-edited CYP1A1 in promoting cancer progression in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Wang,Yan Wu,Ziqi Ding,Xinru Xiao,Yanhua Huang,Zhiguang Liu,Qian Zhang

Experimental dermatology 33:e15009 PubMed38284185

2024

Road-traffic-related air pollution contributes to skin barrier alteration and growth defect of sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Christelle Le Gall-Lanotto,Anthony Verdin,Fabrice Cazier,Adeline Bataille-Savattier,Christelle Guéré,Marie M Dorr,Joachim W Fluhr,Dominique Courcot,Katell Vié,Laurent Misery

Nutrients 16: PubMed38276538

2024

The Garlic Compound, Diallyl Trisulfide, Attenuates Benzo[a]Pyrene-Induced Precancerous Effect through Its Antioxidant Effect, AhR Inhibition, and Increased DNA Repair in Human Breast Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique T Ferguson,Equar Taka,Samia Messeha,Hernan Flores-Rozas,Sarah L Reed,Bryan V Redmond,Karam F A Soliman,Konan J W Kanga,Selina F Darling-Reed

The FEBS journal 290:837-854 PubMed36070107

2022

Fusobacterium nucleatum promotes proliferation in oesophageal squamous cell carcinoma via AHR/CYP1A1 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Haisen Yin,Jianwei Zhang,Hanping Zhang,Qing Li,Hu Qiu,Kunqiao Hong,Wei Wang,Yong Xiao,Baoping Yu

Frontiers in nutrition 9:882222 PubMed35811943

2022

Combinatory Exposure to Urolithin A, Alternariol, and Deoxynivalenol Affects Colon Cancer Metabolism and Epithelial Barrier Integrity .

Applications

Unspecified application

Species

Unspecified reactive species

Julia Groestlinger,Carina Seidl,Elisabeth Varga,Giorgia Del Favero,Doris Marko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CYP1A1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (AB235185)

Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (AB235185)

Western blot - Anti-CYP1A1 antibody (AB235185)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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