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AB230327

Anti-CYP21A2 antibody

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Rabbit Polyclonal CYP21A2 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CYP21A2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CYP21, CYP21B, CYP21A2, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B

4 Images
Western blot - Anti-CYP21A2 antibody (AB230327)
  • WB

Supplier Data

Western blot - Anti-CYP21A2 antibody (AB230327)

All lanes:

Western blot - Anti-CYP21A2 antibody (ab230327) at 1/1000 dilution

All lanes:

MDA-MB-435S (Human ductal carcinoma cell line) cell lysate at 35 µg

Predicted band size: 56 kDa

false

Flow Cytometry (Intracellular) - Anti-CYP21A2 antibody (AB230327)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CYP21A2 antibody (AB230327)

Intracellular flow cytometric analysis of MDA-MB231 (human breast adenocarcinoma cell line) cells labeling CYP21A2 with ab230327 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CYP21A2 antibody (AB230327)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYP21A2 antibody (AB230327)

HEK-293 (human epithelial cell line from embryonic kidney) cells stained for CYP21A2 using ab230327 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in confocal immunofluorescence.

Nucelar counterstain : DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB230327)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB230327)

Formalin-fixed, paraffin-embedded human adrenal tissue stained for CYP21A2 using ab230327 at 1/50 dilution in imunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human CYP21A2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P08686

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP21A2 also known as steroid 21-hydroxylase is an enzyme that plays an important role in steroidogenesis. This enzyme is a part of the cytochrome P450 superfamily and has a molecular mass of approximately 55 kDa. It is mainly expressed in the adrenal cortex where it contributes to the synthesis of glucocorticoids and mineralocorticoids. CYP21A2 is responsible for transforming progesterone and 17-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol respectively by hydroxylation at the 21st carbon position.
Biological function summary

The enzyme activities of CYP21A2 are critical for maintaining homeostasis of steroid hormones. This protein does not function as part of a larger complex but works independently to facilitate the hydroxylation process that is essential for the production of cortisol and aldosterone. These steroid hormones regulate various functions including inflammation control metabolism blood pressure regulation and sodium retention.

Pathways

CYP21A2 is key in the steroid biosynthesis and adrenal corticosteroid synthesis pathways. It operates closely with other enzymes such as CYP11B1 and CYP11B2 which are responsible for further modifications in the production of corticosterone and aldosterone. These pathways ensure the appropriate levels of steroid hormones required for physiological stress response and electrolyte balance.

Mutations or deficiencies in CYP21A2 often lead to conditions like congenital adrenal hyperplasia (CAH) and Addison's disease. In particular CAH stems from inadequate cortisol production resulting in adrenal gland-related complications. CYP21A2 deficiency is connected to heightened androgen levels leading to adrenal hyperplasia. Furthermore the protein's relationship with enzymes like CYP17A1 plays a role in managing the balance between various steroid hormones impacting disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed : 10602386, PubMed : 16984992, PubMed : 22014889, PubMed : 25855791, PubMed : 27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 25855791).
See full target information CYP21A2
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