AB232701

Anti-CYP21A2 antibody

Name: Anti-CYP21A2 antibody (ab232701) | Abcam
Brand: Abcam Limited
SKU: AB232701
Price: 515 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CYP21A2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cyp21 aa 1-300.

View Alternative Names

CYP21, CYP21B, CYP21A2, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB232701)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB232701)

Paraffin-embedded mouse kidney tissue stained for CYP21A2 using ab232701 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB232701)

Paraffin-embedded mouse testis tissue stained for CYP21A2 using ab232701 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-CYP21A2 antibody (AB232701)

All lanes:

Western blot - Anti-CYP21A2 antibody (ab232701) at 3 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 3:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-CYP21A2 antibody (AB232701)

All lanes:

Western blot - Anti-CYP21A2 antibody (ab232701) at 3 µg/mL

All lanes:

Recombinant mouse CYP21B protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Cyp21 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P03940

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232701 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP21A2 also known as steroid 21-hydroxylase is an enzyme that plays an important role in steroidogenesis. This enzyme is a part of the cytochrome P450 superfamily and has a molecular mass of approximately 55 kDa. It is mainly expressed in the adrenal cortex where it contributes to the synthesis of glucocorticoids and mineralocorticoids. CYP21A2 is responsible for transforming progesterone and 17-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol respectively by hydroxylation at the 21st carbon position.

Biological function summary

The enzyme activities of CYP21A2 are critical for maintaining homeostasis of steroid hormones. This protein does not function as part of a larger complex but works independently to facilitate the hydroxylation process that is essential for the production of cortisol and aldosterone. These steroid hormones regulate various functions including inflammation control metabolism blood pressure regulation and sodium retention.

Pathways

CYP21A2 is key in the steroid biosynthesis and adrenal corticosteroid synthesis pathways. It operates closely with other enzymes such as CYP11B1 and CYP11B2 which are responsible for further modifications in the production of corticosterone and aldosterone. These pathways ensure the appropriate levels of steroid hormones required for physiological stress response and electrolyte balance.

Mutations or deficiencies in CYP21A2 often lead to conditions like congenital adrenal hyperplasia (CAH) and Addison's disease. In particular CAH stems from inadequate cortisol production resulting in adrenal gland-related complications. CYP21A2 deficiency is connected to heightened androgen levels leading to adrenal hyperplasia. Furthermore the protein's relationship with enzymes like CYP17A1 plays a role in managing the balance between various steroid hormones impacting disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed : 10602386, PubMed : 16984992, PubMed : 22014889, PubMed : 25855791, PubMed : 27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 25855791).

See full target information CYP21A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:149 PubMed39267003

2024

Branched-chain amino acids supplementation induces insulin resistance and pro-inflammatory macrophage polarization via INFGR1/JAK1/STAT1 signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huaying Huang,Heye Chen,Yu Yao,Xueyong Lou

International journal of molecular sciences 24: PubMed36834842

2023

Characterizing and Quenching Autofluorescence in Fixed Mouse Adrenal Cortex Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Nawar Sakr,Olga Glazova,Liudmila Shevkova,Nikita Onyanov,Samira Kaziakhmedova,Alena Shilova,Maria V Vorontsova,Pavel Volchkov

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYP21A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB232701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB232701)

Western blot - Anti-CYP21A2 antibody (AB232701)

Western blot - Anti-CYP21A2 antibody (AB232701)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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