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AB247085

Anti-CYP21A2 antibody

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(1 Publication)

Rabbit Polyclonal CYP21A2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYP21A2 aa 50-150.

View Alternative Names

CYP21, CYP21B, CYP21A2, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)

Paraffin-embedded human adrenal gland stained for CYP21A2 using ab247085 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)

Paraffin-embedded human kidney tissue stained for CYP21A2 using ab247085 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)

Paraffin-embedded human testis tissue stained for CYP21A2 using ab247085 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody (AB247085)

Paraffin-embedded human pancreas tissue stained for CYP21A2 using ab247085 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-CYP21A2 antibody (AB247085)
  • WB

Supplier Data

Western blot - Anti-CYP21A2 antibody (AB247085)

All lanes:

Western blot - Anti-CYP21A2 antibody (ab247085) at 0.4 µg/mL

All lanes:

Human adrenal gland lysate

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CYP21A2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P08686

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP21A2 also known as steroid 21-hydroxylase is an enzyme that plays an important role in steroidogenesis. This enzyme is a part of the cytochrome P450 superfamily and has a molecular mass of approximately 55 kDa. It is mainly expressed in the adrenal cortex where it contributes to the synthesis of glucocorticoids and mineralocorticoids. CYP21A2 is responsible for transforming progesterone and 17-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol respectively by hydroxylation at the 21st carbon position.
Biological function summary

The enzyme activities of CYP21A2 are critical for maintaining homeostasis of steroid hormones. This protein does not function as part of a larger complex but works independently to facilitate the hydroxylation process that is essential for the production of cortisol and aldosterone. These steroid hormones regulate various functions including inflammation control metabolism blood pressure regulation and sodium retention.

Pathways

CYP21A2 is key in the steroid biosynthesis and adrenal corticosteroid synthesis pathways. It operates closely with other enzymes such as CYP11B1 and CYP11B2 which are responsible for further modifications in the production of corticosterone and aldosterone. These pathways ensure the appropriate levels of steroid hormones required for physiological stress response and electrolyte balance.

Mutations or deficiencies in CYP21A2 often lead to conditions like congenital adrenal hyperplasia (CAH) and Addison's disease. In particular CAH stems from inadequate cortisol production resulting in adrenal gland-related complications. CYP21A2 deficiency is connected to heightened androgen levels leading to adrenal hyperplasia. Furthermore the protein's relationship with enzymes like CYP17A1 plays a role in managing the balance between various steroid hormones impacting disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed : 10602386, PubMed : 16984992, PubMed : 22014889, PubMed : 25855791, PubMed : 27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 25855791).
See full target information CYP21A2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao
View all publications
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