Rabbit Polyclonal CYP21A2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYP21A2.
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- The Journal of steroid biochemistry and molecular 163:12-92016CYP21A2 expression is localized in the developing distal epithelium of the human perinatal lung and is compatible with in situ production and intracrine actions of active glucocorticoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWafae Bouhaddioui et. al.PubMed 27004467
- The Journal of clinical endocrinology and metaboli 95:3418-272010Concomitant mutations in the P450 oxidoreductase and androgen receptor genes presenting with 46,XY disordered sex development and androgenization at adrenarche.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CYP21A2. The exact immunogen used to generate this antibody is proprietary information. Database link P08686
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
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Target data
Function
A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed:10602386, PubMed:16984992, PubMed:22014889, PubMed:25855791, PubMed:27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:25855791).
Alternative names
CYP21, CYP21B, CYP21A2, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B
Recommended products
Rabbit Polyclonal CYP21A2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYP21A2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CYP21A2. The exact immunogen used to generate this antibody is proprietary information. Database link P08686
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP21A2 also known as steroid 21-hydroxylase is an enzyme that plays an important role in steroidogenesis. This enzyme is a part of the cytochrome P450 superfamily and has a molecular mass of approximately 55 kDa. It is mainly expressed in the adrenal cortex where it contributes to the synthesis of glucocorticoids and mineralocorticoids. CYP21A2 is responsible for transforming progesterone and 17-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol respectively by hydroxylation at the 21st carbon position.
- Biological function summary
The enzyme activities of CYP21A2 are critical for maintaining homeostasis of steroid hormones. This protein does not function as part of a larger complex but works independently to facilitate the hydroxylation process that is essential for the production of cortisol and aldosterone. These steroid hormones regulate various functions including inflammation control metabolism blood pressure regulation and sodium retention.
- Pathways
CYP21A2 is key in the steroid biosynthesis and adrenal corticosteroid synthesis pathways. It operates closely with other enzymes such as CYP11B1 and CYP11B2 which are responsible for further modifications in the production of corticosterone and aldosterone. These pathways ensure the appropriate levels of steroid hormones required for physiological stress response and electrolyte balance.
- Associated diseases and disorders
Mutations or deficiencies in CYP21A2 often lead to conditions like congenital adrenal hyperplasia (CAH) and Addison's disease. In particular CAH stems from inadequate cortisol production resulting in adrenal gland-related complications. CYP21A2 deficiency is connected to heightened androgen levels leading to adrenal hyperplasia. Furthermore the protein's relationship with enzymes like CYP17A1 plays a role in managing the balance between various steroid hormones impacting disease outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-CYP21A2 antibody (ab64887)
All lanes: Western blot - Anti-CYP21A2 antibody (ab64887) at 1/500 dilution
Lane 1: extracts from 293 cells (5-30µg total protein)
Lane 2: extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 56 kDa
Observed band size: 40 kDa, 56 kDa
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