Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)

Rabbit Recombinant Monoclonal CYP21A2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (AB324020), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Tested	Tested
Rat	Tested	Tested
Transfected cell line - Human	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Target data

See full target information CYP21A2

Function

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed:10602386, PubMed:16984992, PubMed:22014889, PubMed:25855791, PubMed:27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:25855791).

Additional Targets

Cyp21, Cyp21

Alternative names

CYP21, CYP21B, CYP21A2, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B

Rabbit Recombinant Monoclonal CYP21A2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR29787-27
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab324020 is the carrier free version of Anti-CYP21A2 antibody [EPR29787-27] ab324019.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Western blot staining using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver, placenta
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CYP21A2 antibody [EPR29787-27] (Anti-CYP21A2 antibody [EPR29787-27] ab324019) at 1/1000 dilution
Lane 1: Human adrenal gland tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Mouse adrenal gland tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Mouse placenta tissue lysate at 20 µg
Lane 6: Rat adrenal gland tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
Lane 8: Rat placenta tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa, 36 kDa
Exposure time: 59s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human adrenal gland using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human adrenal gland.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse adrenal gland using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse adrenal gland.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat adrenal gland using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat adrenal gland tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat adrenal gland.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human pancreas.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat liver using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in rat liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP21A2 antibody [EPR29787-27] - BSA and Azide free (ab324020)
CYP21A2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-CYP21A2 antibody
This data was developed using Anti-CYP21A2 antibody [EPR29787-27] ab324019, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human CYP21A2 expression vector containing a Myc-His tag and (B) HEK-293T transfected with empty vector containing a Myc-His tag labeling CYP21A2 with Anti-CYP21A2 antibody [EPR29787-27] ab324019 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) HEK-293T transfected with a human CYP21A2 expression vector containing a Myc-His tag, no signal in (B) HEK-293T transfected with empty vector containing a Myc-His tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins