Rabbit Polyclonal CYP26C1 antibody. N-terminal. Suitable for WB and reacts with Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP26C1 aa 1-250.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYP26C1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q6V0L0
Reactivity data
Select an application| WB | |
|---|---|
| Zebrafish | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals (PubMed:14532297). RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (Probable). Catalyzes the oxidation of atRA primarily at C-4 (PubMed:14532297). Oxidation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination, thereby contributes to the regulation of atRA homeostasis and signaling (Probable). Able to metabolize other RAs such as 9-cis with high efficiency (PubMed:14532297). Can oxidize all-trans-13,14-dihydroretinoate (DRA) to metabolites which could include all-trans-4-oxo-DRA, all-trans-4-hydroxy-DRA, all-trans-5,8-epoxy-DRA, and all-trans-18-hydroxy-DRA (By similarity). Shares sequence similarity with other CYP26 family members, but has higher affinity to 9-cis-RA and is much less sensitive to the inhibitory effects of ketoconazole (PubMed:14532297). In cooperation with Cyp26a1, contributes to the CNS patterning and the development of regions of higher visual acuity (By similarity).
Alternative names
Cytochrome P450 26C1, CYP26C1, CYP26C1
Recommended products
Rabbit Polyclonal CYP26C1 antibody. N-terminal. Suitable for WB and reacts with Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP26C1 aa 1-250.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYP26C1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q6V0L0
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP26C1 also known as Cytochrome P450 26C1 is an enzyme of the Cytochrome P450 superfamily responsible for the metabolism of retinoic acid. This enzyme helps maintain retinoic acid homeostasis by oxidizing it into hydroxylated forms. CYP26C1 has a molecular mass of approximately 57 kDa. The expression of CYP26C1 mainly occurs in the brain skin and thyroid gland where it plays a role in managing local concentrations of retinoic acid which is vital for tissue morphogenesis differentiation and cell proliferation control.
- Biological function summary
The protein supports the regulation of retinoic acid levels within cells to ensure proper gene expression during developmental processes. While not part of a larger enzyme complex CYP26C1's activity influences retinoid signaling pathways which impact a wide array of physiological responses including cell growth and differentiation. The activity of CYP26C1 is important for proper embryonic development and its dysfunction may lead to developmental abnormalities.
- Pathways
CYP26C1 is involved in both the retinoic acid metabolism pathway and the signaling pathway. The enzyme helps metabolize all-trans retinoic acid a signaling molecule essential for transcription regulation. Through these pathways CYP26C1 interacts with several other proteins including CYP26A1 and CYP26B1 both of which also regulate retinoic acid levels ensuring its bioavailability is tightly controlled during critical periods of development.
- Associated diseases and disorders
CYP26C1 holds connections with conditions like congenital cerebellar ataxia and spina bifida. Disruptions in CYP26C1's enzymatic activity can lead to imbalances in retinoic acid contributing to these developmental disorders. Furthermore studies have linked CYP26C1's interactions with proteins like Retinoic Acid Receptor (RAR) to the pathogenesis of retinoic acid-responsive cancers highlighting its impact on both normal development and disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-CYP26C1 antibody - N-terminal (ab211115)
7.5% SDS PAGE
All lanes: Western blot - Anti-CYP26C1 antibody - N-terminal (ab211115) at 1/1000 dilution
All lanes: zebrafish liver whole cell lysate at 30 µg
Predicted band size: 57 kDa
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