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AB126785

Anti-CYP27A1 antibody [EPR7529]

5

(6 Reviews)

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(43 Publications)

Anti-CYP27A1 antibody [EPR7529] (ab126785) is a rabbit monoclonal antibody detecting CYP27A1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

CYP27, CYP27A1, Cytochrome P-450C27/25, Cytochrome P450 27, Sterol 27-hydroxylase, Vitamin D(3) 25-hydroxylase

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (AB126785)

ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (AB126785)

ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CYP27A1 antibody [EPR7529] (AB126785)

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (AB126785)

ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.

Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • WB

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Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)

All lanes:

Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

Caco 2 lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

Mouse liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

false

Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • WB

Lab

Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Exposure time: 180s

Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • WB

Lab

Western blot - Anti-CYP27A1 antibody [EPR7529] (AB126785)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Exposure time: 180s

OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (AB126785)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (AB126785)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free

  • 578 PE

    PE Anti-CYP27A1 antibody [EPR7529]

  • 660 APC

    APC Anti-CYP27A1 antibody [EPR7529]

  • HRP

    HRP Anti-CYP27A1 antibody [EPR7529]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CYP27A1 antibody [EPR7529]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CYP27A1 antibody [EPR7529]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CYP27A1 antibody [EPR7529]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CYP27A1 antibody [EPR7529]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CYP27A1 antibody [EPR7529]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CYP27A1 antibody [EPR7529]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7529

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/120", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-CYP27A1 antibody [EPR7529] (ab126785) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CYP27A1?
Anti-CYP27A1 [EPR7529] (ab126785) specifically detects a band for CYP27A1 (UniProt: Q02318) at a molecular weight of 60kDa.

Trusted by the scientific community
Anti-CYP27A1 [EPR7529] (ab126785) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-CYP27A1 [EPR7529] (ab126785) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR7529] also available for your convenience: ab126785, Carrier free - ab214800, PE - ab305862, APC - ab305863, HRP - ab305864, Alkaline Phosphatase - ab308834, Alexa Fluor® 488 - ab309770, Alexa Fluor® 647 - ab310141, Alexa Fluor® 594 - ab310565, Alexa Fluor® 555 - ab312094, Alexa Fluor® 568 - ab312574, Alexa Fluor® 750 - ab321015

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP27A1 also known as sterol 27-hydroxylase is a member of the cytochrome P450 superfamily. It functions as a mitochondrial enzyme with a mass of approximately 59 kDa localized in the liver and various other tissues. CYP27A1 hydroxylates its substrates playing an important role in cholesterol metabolism. This process involves the conversion of cholesterol to bile acids an essential step in enabling the excretion of cholesterol from the body. High expression levels of CYP27A1 can be observed in hepatic tissue reflecting its central role in lipid metabolism.
Biological function summary

CYP27A1 participates in cholesterol catabolism. While it does not form large protein complexes it interacts with other enzymes in metabolic pathways. This enzyme initiates the acidic pathway of bile acid synthesis by converting cholesterol into 27-hydroxycholesterol. These transformations are important for maintaining cholesterol homeostasis and for producing bile acids that aid in fat digestion and nutrient absorption.

Pathways

CYP27A1 is central to the bile acid biosynthesis and cholesterol metabolism pathways. It collaborates with other enzymes such as CYP7A1 which is known for initiating the classic pathway of bile acid synthesis. Together they regulate cholesterol levels by metabolizing cholesterol into bile acids. Such pathways are critical in preventing cholesterol accumulation which could lead to various metabolic disorders.

Impairment of CYP27A1 function links to cerebrotendinous xanthomatosis (CTX) marked by the accumulation of cholestanol in tissues leading to neurological dysfunction. Mutations in the CYP27A1 gene disrupt cholesterol metabolism resulting in the accumulation of cholesterol derivatives. Furthermore there is a connection to cardiovascular disease due to the role of CYP27A1 in maintaining cholesterol balance linked with proteins like ApoE impacting lipid transport.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (PubMed : 12077124, PubMed : 21411718, PubMed : 28190002, PubMed : 9660774). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways (PubMed : 11412116, PubMed : 1708392, PubMed : 2019602, PubMed : 7915755, PubMed : 9186905, PubMed : 9660774, PubMed : 9790667). May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (PubMed : 12077124, PubMed : 9660774). Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation (PubMed : 28190002). Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium (PubMed : 21411718). May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PubMed : 15465040).
See full target information CYP27A1
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40846326

2025

Multiomics integration analysis identifies tumor cell-derived MIF as a therapeutic target and potentiates anti-PD-1 therapy in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Chen,Yan Liao,Hao Yao,Yutong Zou,Ji Fang,Jiongfeng Zhang,Zehao Guo,Jian Tu,Junkai Chen,Zijun Huo,Lili Wen,Xianbiao Xie

Chinese medicine 20:68 PubMed40414923

2025

ZeXieYin formula alleviates atherosclerosis by regulating SBAs levels through the FXR/FGF15 pathway and restoring intestinal barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Shihan Zhou,Shangbo Hua,Xinyi Chen,Meiling Ni,Jing Liu,Yanqing Wang,Wanning Wu,Anni Ding,Zizhen Qin,Xinyu Yang,Xiaowei Chen,Boran Zhu,Haoxin Wu

iScience 28:111804 PubMed39995863

2025

Hepatic Lamp2a deficiency promotes inflammation of murine autoimmune cholangitis via affecting bile acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qingling Fan,Guanya Guo,Yinan Hu,Yi Lu,Rui Su,Jiaqi Yang,Erzhuo Xia,Shuoyi Ma,Miao Zhang,Jingbo Wang,Ting Li,Ying Han

Experimental and therapeutic medicine 28:361 PubMed39071912

2024

Sodium sulphate ameliorates hypercholesterolemia via the upregulation of in hepatocytes and alleviates hepatic insulin resistance via the downregulation of in mice with high cholesterol diets.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Yang,Siping Yu,Hedong Rong,Zili Lei,Changyuan Yang,Huijuan Wu,Tianle Zhang,Fei Yang,Ya Nie,Lei Chen,Qing Hu,Qi Song,Jiao Guo

Cellular and molecular life sciences : CMLS 81:226 PubMed38775844

2024

Cholesterol neutralized vemurafenib treatment by promoting melanoma stem-like cells via its metabolite 27-hydroxycholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Wang,Feiliang Zhong,Tingting Chen,Hongbo Wang,Weifang Wang,Hongkai Jin,Chouyang Li,Xuan Guo,Ying Liu,Yu Zhang,Bo Li

Journal of lipid research 64:100479 PubMed37981011

2023

27-Hydroxylation of oncosterone by CYP27A1 switches its activity from pro-tumor to anti-tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Silia Ayadi,Silvia Friedrichs,Regis Soulès,Laly Pucheu,Dieter Lütjohann,Sandrine Silvente-Poirot,Marc Poirot,Philippe de Medina

BMC biotechnology 23:43 PubMed37789318

2023

Engineered FGF19 protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Shi,Tiantian Zhao,Lei Huang,Xiaomin Pan,Tianzhen Wu,Xin Feng,Taoli Chen,Jiamin Wu,Jianlou Niu

Hepatology communications 7: PubMed37556375

2023

Bile acids promote the development of HCC by activating inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Chen,Ming Ding,Liyan Ji,Jingjing Yao,Yajuan Guo,Wenxin Yan,Shaofang Yu,Qinghong Shen,Min Huang,Yaqiu Zheng,Yuefang Lin,Ying Wang,Zhongqiu Liu,Linlin Lu,Xin Jin

Nature communications 14:4101 PubMed37491347

2023

Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Yu,Lin Xu,Haiyan Chu,Jun Peng,Anastasia Sacharidou,Hsi-Hsien Hsieh,Ada Weinstock,Sohaib Khan,Liqian Ma,José Gabriel Barcia Durán,Jeffrey McDonald,Erik R Nelson,Sunghee Park,Donald P McDonnell,Kathryn J Moore,Lily Jun-Shen Huang,Edward A Fisher,Chieko Mineo,Linzhang Huang,Philip W Shaul

The Journal of biological chemistry 299:105035 PubMed37442234

2023

Mitochondrial cytochrome P450 1B1 is involved in pregnenolone synthesis in human brain cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqi Christina Lin,Garett Cheung,Zeyu Zhang,Vassilios Papadopoulos
View all publications
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