Rabbit Recombinant Monoclonal CYP27B1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Recombinant fragment, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106311.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123562.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)")
![Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunoprecipitation-img54545.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30163.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)")
![Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--western-blot-img420772.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Predicted | Expected | Predicted |
| Rat | Expected | Expected | Tested |
| Recombinant fragment | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Target data
Function
A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed:10518789, PubMed:10566658, PubMed:12050193, PubMed:22862690, PubMed:9486994). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed:10518789, PubMed:22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed:10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:22862690).
Alternative names
CYP1ALPHA, CYP27B, CYP27B1, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, VD3 1A hydroxylase
Recommended products
Rabbit Recombinant Monoclonal CYP27B1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Recombinant fragment, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20271
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238978 is the carrier-free version of Anti-CYP27B1 antibody [EPR20271] ab206655.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome P450 family 27 subfamily B member 1 (CYP27B1) also known as 25-hydroxyvitamin D3 1-alpha-hydroxylase plays a mechanical role in the hydroxylation of 25-hydroxyvitamin D3 into the active form 125-dihydroxyvitamin D3 (calcitriol). This enzyme is approximately 56 kDa in size. Expression is notably high in the kidneys which is the primary site for its enzymatic activity but it is also present in several other tissues including skin lungs and immune cells.
- Biological function summary
The active form of vitamin D produced by CYP27B1 is essential for maintaining mineral homeostasis and bone health. The enzyme is not traditionally part of a larger protein complex but works closely with vitamin D binding proteins to interact with its substrates. The active vitamin D influences calcium and phosphate balance in the body which has implications for overall bone integrity and immune system regulation.
- Pathways
CYP27B1 plays a significant role in the vitamin D metabolic pathway and the calcium signaling pathway. It functions upstream of the vitamin D receptor (VDR) that helps control calcium absorption in the gut. CYP24A1 another related cytochrome P450 enzyme participates in degrading the active form of vitamin D maintaining an important balance within the system. Together these pathways and proteins help regulate calcium levels critical to many physiological processes.
- Associated diseases and disorders
CYP27B1 has notable connections to rickets and osteoporosis. Mutations in the CYP27B1 gene can lead to reduced or absent enzyme activity resulting in Vitamin D-dependent rickets type 1 characterized by impaired bone mineralization. Its activity also affects osteoporosis a condition of reduced bone density. The balance between CYP27B1 and CYP24A1 is important in understanding the pathogenesis of these bone-related disorders where altered vitamin D metabolism can contribute to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106311.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CYP27B1 with Anti-CYP27B1 antibody [EPR20271] ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Strong cytoplasmic staining on distal convoluted tubules of human kidney. Weaker cytoplasmic staining on proximal convoluted tubules [PMID: 23416105] [PMID: 12050281].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP27B1 antibody [EPR20271] ab206655).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123562.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CYP27B1 with Anti-CYP27B1 antibody [EPR20271] ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules [PMID: 23416105] [PMID: 12050281].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP27B1 antibody [EPR20271] ab206655).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunoprecipitation-img54545.jpg "Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)")
Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)
CYP27B1 was immunoprecipitated from 0.35 mg of MeWo (Human malignant melanoma cell line) whole cell lysate with Anti-CYP27B1 antibody [EPR20271] ab206655 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-CYP27B1 antibody [EPR20271] ab206655 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: MeWo whole cell lysate, 10 μg (Input).
Lane 2: Anti-CYP27B1 antibody [EPR20271] ab206655 IP in MeWo whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CYP27B1 antibody [EPR20271] ab206655 in MeWo whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP27B1 antibody [EPR20271] ab206655).
All lanes: Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] (Anti-CYP27B1 antibody [EPR20271] ab206655)
Predicted band size: 57 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30163.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CYP27B1 with Anti-CYP27B1 antibody [EPR20271] ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on rat kidney tubules [PMID: 23416105] [PMID: 12050281].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP27B1 antibody [EPR20271] ab206655).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978), expandable thumbnail](https://content.abcam.com/products/images/cyp27b1-antibody-epr20271-bsa-and-azide-free-ab238978--western-blot-img420772.jpg "Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)")
Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (ab238978)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP27B1 antibody [EPR20271] ab206655).Lane 1: Western blot - Anti-CYP27B1 antibody [EPR20271] (Anti-CYP27B1 antibody [EPR20271] ab206655) at 1/1000 dilution
Lane 2: Anti-His-tag antibody at 1/200 dilution
All lanes: Recombinant CYP27B1 protein
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 56 kDa
Exposure time: 3s
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