AB238978

Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CYP27B1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Recombinant fragment, Mouse samples. Cited in 1 publication.

View Alternative Names

CYP1ALPHA, CYP27B, CYP27B1, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, VD3 1A hydroxylase

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CYP27B1 with ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Strong cytoplasmic staining on distal convoluted tubules of human kidney. Weaker cytoplasmic staining on proximal convoluted tubules [PMID : 23416105] [PMID : 12050281].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

CYP27B1 was immunoprecipitated from 0.35 mg of MeWo (Human malignant melanoma cell line) whole cell lysate with ab206655 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206655 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : MeWo whole cell lysate, 10 μg (Input).

Lane 2 : ab206655 IP in MeWo whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206655 in MeWo whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206655).

All lanes:

Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] (<a href='/en-us/products/primary-antibodies/cyp27b1-antibody-epr20271-ab206655'>ab206655</a>)

Predicted band size: 57 kDa

Observed band size: 45 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CYP27B1 with ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules [PMID : 23416105] [PMID : 12050281].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CYP27B1 with ab206655 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat kidney tubules [PMID : 23416105] [PMID : 12050281].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206655).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206655).

Lane 1:

Western blot - Anti-CYP27B1 antibody [EPR20271] (<a href='/en-us/products/primary-antibodies/cyp27b1-antibody-epr20271-ab206655'>ab206655</a>) at 1/1000 dilution

Lane 2:

Anti-His-tag antibody at 1/200 dilution

All lanes:

Recombinant CYP27B1 protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 56 kDa

false

Exposure time: 3s

Unconjugated

Anti-CYP27B1 antibody [EPR20271]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20271

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab238978 is the carrier-free version of ab206655.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 family 27 subfamily B member 1 (CYP27B1) also known as 25-hydroxyvitamin D3 1-alpha-hydroxylase plays a mechanical role in the hydroxylation of 25-hydroxyvitamin D3 into the active form 125-dihydroxyvitamin D3 (calcitriol). This enzyme is approximately 56 kDa in size. Expression is notably high in the kidneys which is the primary site for its enzymatic activity but it is also present in several other tissues including skin lungs and immune cells.

Biological function summary

The active form of vitamin D produced by CYP27B1 is essential for maintaining mineral homeostasis and bone health. The enzyme is not traditionally part of a larger protein complex but works closely with vitamin D binding proteins to interact with its substrates. The active vitamin D influences calcium and phosphate balance in the body which has implications for overall bone integrity and immune system regulation.

Pathways

CYP27B1 plays a significant role in the vitamin D metabolic pathway and the calcium signaling pathway. It functions upstream of the vitamin D receptor (VDR) that helps control calcium absorption in the gut. CYP24A1 another related cytochrome P450 enzyme participates in degrading the active form of vitamin D maintaining an important balance within the system. Together these pathways and proteins help regulate calcium levels critical to many physiological processes.

CYP27B1 has notable connections to rickets and osteoporosis. Mutations in the CYP27B1 gene can lead to reduced or absent enzyme activity resulting in Vitamin D-dependent rickets type 1 characterized by impaired bone mineralization. Its activity also affects osteoporosis a condition of reduced bone density. The balance between CYP27B1 and CYP24A1 is important in understanding the pathogenesis of these bone-related disorders where altered vitamin D metabolism can contribute to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed : 10518789, PubMed : 10566658, PubMed : 12050193, PubMed : 22862690, PubMed : 9486994). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed : 10518789, PubMed : 22862690). Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed : 10518789). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed : 22862690).

See full target information CYP27B1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1579843 PubMed40661777

2025

Analysis of the molecular subtypes and prognostic models of anoikis-related genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Kang Hou,Jifeng Zhang,Xiaodong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunoprecipitation - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Western blot - Anti-CYP27B1 antibody [EPR20271] - BSA and Azide free (AB238978)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise