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AB56069

Anti-CYP2A6 antibody [F16 P2 D8]

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(2 Publications)

Mouse Monoclonal CYP2A6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYP2A6 conjugated to Ovalbumin.

View Alternative Names

CYP2A3, CYP2A6, Cytochrome P450 2A6, CYPIIA6, Coumarin 7-hydroxylase, Cytochrome P450 IIA3, Cytochrome P450(I)

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2A6 antibody [F16 P2 D8] (AB56069)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2A6 antibody [F16 P2 D8] (AB56069)

This image shows normal human liver tissue stained by ab56069 at 1µg/ml.

Western blot - Anti-CYP2A6 antibody [F16 P2 D8] (AB56069)
  • WB

Unknown

Western blot - Anti-CYP2A6 antibody [F16 P2 D8] (AB56069)

All lanes:

Western blot - Anti-CYP2A6 antibody [F16 P2 D8] (ab56069) at 1 µg/mL

All lanes:

Recombinant cytochrome p450

Predicted band size: 57 kDa

Observed band size: 54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F16 P2 D8

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CYP2A6 conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P11509

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP2A6 also known as cytochrome P450 2A6 is an enzyme with a mass of approximately 56 kDa. It is a member of the cytochrome P450 superfamily and is expressed mainly in the liver with some expression in the respiratory tract. CYP2A6 is involved in the oxidation of several drugs and foreign chemicals. It functions by catalyzing the hydroxylation of various substrates which aids in their metabolism and clearance from the body. Despite its primary presence in hepatic tissue its expression can occur in other extrahepatic tissues as well.
Biological function summary

CYP2A6 plays a significant role in phase I metabolism as part of the cytochrome P450 enzyme complex. This enzyme contributes to the metabolic activation and detoxification of many xenobiotic substances including nicotine and coumarin. Through its enzymatic action CYP2A6 influences the pharmacokinetics of several drugs impacting their absorption distribution metabolism and excretion. It also contributes to the interindividual variability seen in the metabolism of drugs affecting both therapeutic outcomes and toxicity risks.

Pathways

CYP2A6 plays an essential role in drug metabolism and the detoxification pathway. It is heavily involved in the metabolism of nicotine by converting it to cotinine therefore playing a prominent role in nicotine addiction and dependence. CYP2A6 is also associated with the synthesis of steroid hormones. Through these pathways it interacts with other cytochrome P450 enzymes like CYP2A7 as well as enzymes involved in the drug metabolism processes.

CYP2A6 is linked with several conditions primarily due to its role in drug metabolism. Altered activity of CYP2A6 can affect the risk of nicotine addiction impacting individuals' susceptibility to tobacco dependence. Furthermore variations in CYP2A6 activity are associated with susceptibility to certain cancers due to changes in the activation of procarcinogens. The enzyme's involvement in liver cancer emphasizes the importance of understanding its interaction with other CYP enzymes notably CYP2A6/7. This understanding suggests potential pathways for therapeutic targeting in diseases linked to metabolic dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
See full target information CYP2A6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Inhalation toxicology 27:767-77 PubMed26572092

2015

Gene expression and immunochemical localization of major cytochrome P450 drug-metabolizing enzymes in bovine nasal olfactory and respiratory mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Varsha Dhamankar,Mahfoud Assem,Maureen D Donovan

Toxicology and applied pharmacology 289:30-9 PubMed26343999

2015

Tumour suppressor protein p53 regulates the stress activated bilirubin oxidase cytochrome P450 2A6.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Hu,Ting Yu,Satu Arpiainen,Matti A Lang,Jukka Hakkola,A'edah Abu-Bakar
View all publications
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