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Rabbit Recombinant Monoclonal CP2CJ antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (AB236063), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (AB236063), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (AB236063), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (AB236063), expandable thumbnail
  • OI-RD Scanning - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (AB236063), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Predicted
Mouse
Expected
Tested
Rat
Expected
Predicted

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat, Human
Dilution info
-
Notes

-

Target data

Function

A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) (PubMed:18577768, PubMed:19965576, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:18577768, PubMed:19965576, PubMed:20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates PUFA specifically at the omega-1 position (PubMed:18577768). Catalyzes the epoxidation of double bonds of PUFA (PubMed:19965576, PubMed:20972997). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794). Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine. Hydroxylates fenbendazole at the 4' position (PubMed:23959307).

Alternative names

Cytochrome P450 2C19, (R)-limonene 6-monooxygenase, (S)-limonene 6-monooxygenase, (S)-limonene 7-monooxygenase, CYPIIC17, CYPIIC19, Cytochrome P450-11A, Cytochrome P450-254C, Fenbendazole monooxygenase (4'-hydroxylating), Mephenytoin 4-hydroxylase, CYP2C19

Recommended products

Rabbit Recombinant Monoclonal CP2CJ antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6576
Purification technique
Affinity purification Protein A
Specificity

This antibody is very likely to be cross-reactive with many Cytochrome P450 family members. According to the blast result, in human species, the immunogen has 100% homology with CYP2C9, CYP2A6 and CYP2A7, 85.7% with CYP2C18, 78.6% with CYP2A13 and CYP2C8. CYP2C19 doesn`t exist in mouse and rat species. The immunogen shares 100% homology with mouse CYP2A4, CYP2A5, CYP2A12, CYP2C29, CYP2C70 and rat CYP2A3, CYP2C6, CYP2C55, 92.9% with mouse CYP2C37, CYP2C50, CYP2C55 and rat CYP2C11, CYP2C13.

Dissociation constant
1.24 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab236063 is the carrier-free version of Anti-CYP2C19 antibody [EPR6576] ab137015.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CYP2C19 also referred to as cytochrome P450 2C19 is an enzyme that belongs to the cytochrome P450 superfamily. This protein has a molecular mass of approximately 56 kDa. CYP2C19 is primarily expressed in the liver but also appears in the intestine. The enzyme plays a significant mechanical role in the oxidative metabolism of various substrates including both endogenous compounds and exogenous drugs. The chemical reactions it facilitates involve the oxidation of organic substances.

Biological function summary

Cytochrome P450 2C19 is responsible for metabolizing drugs such as proton pump inhibitors antiepileptic drugs and some antidepressants. This enzyme is part of a larger enzyme family that contributes to the detoxification processes and biosynthesis of steroid hormones. It acts by catalyzing the oxidative transformation of compounds which influences drug activation and clearance. Its substrate specificity includes many significant pharmacological agents.

Pathways

CYP2C19 is integrally involved in drug metabolism pathways particularly the Phase I metabolism. It cooperates with enzymes like CYP2C9 and CYP3A4 within these pathways performing hydrolytic and oxidative reactions that transform lipophilic drugs into more water-soluble derivatives. Consequently this activity prepares compounds for further modification in Phase II metabolism facilitating excretion from the body.

Associated diseases and disorders

CYP2C19 is implicated in conditions such as cardiovascular disease and adverse drug reactions. Genetic polymorphisms within the CYP2C19 gene lead to varied metabolic capacities affecting the efficacy and safety of therapeutics especially antiplatelet drugs like clopidogrel. Additionally alterations in CYP2C19 activity are associated with altered drug metabolism which may result in interactions with proteins such as P-glycoprotein and other cytochrome P450 enzymes affecting therapeutic outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling CYP2C19 with Purified Anti-CYP2C19 antibody [EPR6576] ab137015 at 1:1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP2C19 antibody [EPR6576] ab137015).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling CYP2C19 with Purified Anti-CYP2C19 antibody [EPR6576] ab137015 at 1:1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP2C19 antibody [EPR6576] ab137015).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CYP2C19 with Purified Anti-CYP2C19 antibody [EPR6576] ab137015 at 1:1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP2C19 antibody [EPR6576] ab137015).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labelling CYP2C19 with Anti-CYP2C19 antibody [EPR6576] ab137015 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CYP2C19 antibody [EPR6576] ab137015).

  • OI-RD Scanning - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063), expandable thumbnail

    OI-RD Scanning - Anti-CYP2C19 antibody [EPR6576] - BSA and Azide free (ab236063)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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