AB151996

Anti-CYP2J2 antibody

Name: Anti-CYP2J2 antibody (ab151996) | Abcam
Brand: Abcam Limited
SKU: AB151996
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal CYP2J2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Cytochrome P450 2J2 aa 200-500.

View Alternative Names

Cytochrome P450 2J2, Albendazole monooxygenase (hydroxylating), Albendazole monooxygenase (sulfoxide-forming), Arachidonic acid epoxygenase, CYPIIJ2, Hydroperoxy icosatetraenoate isomerase, CYP2J2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2J2 antibody (AB151996)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2J2 antibody (AB151996)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CYP2J2 with ab151996 at 1/500 dilution.

Unknown

Western blot - Anti-CYP2J2 antibody (AB151996)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CYP2J2 antibody (ab151996) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Cytochrome P450 2J2 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P51589

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP2J2 also known as cytochrome P450 2J2 is an enzyme belonging to the cytochrome P450 superfamily. It catalyzes the metabolism of arachidonic acid to epoxyeicosatrienoic acids (EETs) which are signalling molecules. CYP2J2 has a mass of approximately 57 kDa and is prominently expressed in the heart although expression also occurs in the liver intestine and other organs. The enzyme's activity involves the oxidation of various substrates including certain drugs contributing to their metabolism and elimination.

Biological function summary

CYP2J2 contributes to cardiovascular function regulation by producing EETs. EETs have anti-inflammatory and vasodilatory effects which maintain vascular health. This enzyme does not usually form part of larger complexes but can influence other biological processes independently. The regulation of blood pressure and prevention of inflammation highlight the vascular role of CYP2J2 through EETs.

Pathways

CYP2J2 plays a significant role in the arachidonic acid cascade. This pathway involves the conversion of arachidonic acid into various eicosanoids each with distinct biological functions. CYP2J2 directly interacts with arachidonic acid modifying it into EETs differing from the action of other cytochrome P450 enzymes like CYP2C8. These enzymes collaboratively participate in complex lipid signalling pathways which maintain cardiovascular health and homeostasis.

CYP2J2 has a role in cardiac disorders such as ischemia and hypertension. Increased activity or expression of CYP2J2 can provide protective effects against cardiac injury due to the vasodilatory and anti-inflammatory properties of EETs. Related proteins like CYP2C9 also participate in similar roles but with distinct regional effects in tissues. Variations in CYP2J2 expression or mutations can influence susceptibility to cardiovascular diseases highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system (PubMed : 19965576, PubMed : 8631948). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 19965576, PubMed : 8631948). Catalyzes the epoxidation of double bonds of PUFA (PubMed : 19965576, PubMed : 8631948). Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools (PubMed : 8631948). In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent (PubMed : 19737933). Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone (PubMed : 19923256). Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole (PubMed : 23959307). Catalyzes the sulfoxidation of fenbedazole (PubMed : 19923256).

See full target information Cytochrome P450 2J2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1386528 PubMed38590527

2024

The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Zdenek Tauber,Adela Burianova,Katerina Koubova,Max Mrstik,Marie Jirkovska,Katerina Cizkova

Redox biology 63:102765 PubMed37269686

2023

EETs alleviate alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress through the Trim25/Keap1/Nrf2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Yu Zhang,Wen-Jing Zhong,Yu-Biao Liu,Jia-Xi Duan,Nan Jiang,Hui-Hui Yang,Sheng-Chao Ma,Ling Jin,Jie-Ru Hong,Yong Zhou,Cha-Xiang Guan

Oncology letters 23:180 PubMed35464300

2022

Artemether inhibits proliferation, invasion and migration of hepatocellular carcinoma cells via targeting of CYP2J2.

Applications

Unspecified application

Species

Unspecified reactive species

Xionglin Zhu,Mei Yang,Zhiling Song,Guangbing Yao,Qifeng Shi

Communications biology 4:138 PubMed33514783

2021

Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Furihata,Shingo Takada,Naoya Kakutani,Satoshi Maekawa,Masaya Tsuda,Junichi Matsumoto,Wataru Mizushima,Arata Fukushima,Takashi Yokota,Nobuyuki Enzan,Shouji Matsushima,Haruka Handa,Yoshizuki Fumoto,Junko Nio-Kobayashi,Toshihiko Iwanaga,Shinya Tanaka,Hiroyuki Tsutsui,Hisataka Sabe,Shintaro Kinugawa

Frontiers in oncology 10:574277 PubMed33330047

2020

Targeting CYP2J2 to Enhance the Anti-Glioma Efficacy of Cannabinoid Receptor 2 Stimulation by Inhibiting the Pro-Angiogenesis Function of M2 Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Lei,Xuezhu Chen,Yulian Quan,Yihao Tao,Junlong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CYP2J2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2J2 antibody (AB151996)

Western blot - Anti-CYP2J2 antibody (AB151996)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise