JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137634

Anti-CYP2R1 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal CYP2R1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Vitamin D 25-hydroxylase aa 50-400.

View Alternative Names

Vitamin D 25-hydroxylase, Cytochrome P450 2R1, CYP2R1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2R1 antibody (AB137634)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2R1 antibody (AB137634)

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, labelling CYP2R1 using ab137634 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CYP2R1 antibody (AB137634)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CYP2R1 antibody (AB137634)

Immunofluorescence analysis of methanol-fixed HeLa cells, labelling CYP2R1 using ab137634 at 1/200 dilution. lower panel represents cells costained with Hoechst 33342.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Vitamin D 25-hydroxylase aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q6VVX0

style
left-column

Alternative Products

Primary Antibodies

AB239217

Anti-CYP2R1 antibody

Western blot - Anti-CYP2R1 antibody (AB239217)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB314119

Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-PTHLH antibody [EPR26606-236] - BSA and Azide free (AB314119)

  • 0
  • 0
Primary Antibodies

AB183910

Anti-Osteoprotegerin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody (AB183910)

  • 0
  • 0
Primary Antibodies

AB221115

Anti-Retinoid X Receptor beta/RXRB antibody

Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor beta/RXRB antibody (AB221115)

  • 0
  • 0
Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP2R1 also known as Cytochrome P450 2R1 functions as a microsomal enzyme playing a major role in the hydroxylation of vitamin D. It catalyzes the conversion of vitamin D to 25-hydroxyvitamin D a form needed for further activation to the biologically active version. The protein has a known molecular mass of about 55 kDa. CYP2R1 is expressed in the liver which is important for its function owing to the liver's role in metabolizing vitamin D.
Biological function summary

CYP2R1 participates in the metabolic pathway to synthesize the active form of vitamin D. Although not part of a larger complex its activity serves a critical function in maintaining calcium homeostasis and bone health by mediating vitamin D metabolism. This process enables the regulation of calcium and phosphate essential for proper bone formation and maintenance.

Pathways

CYP2R1 plays an integral role in the vitamin D signaling pathway which is important for its metabolic conversion. Another related pathway is the cytochrome P450 metabolic pathway where CYP enzymes collaborate in synthesis and degradation processes. Within these pathways CYP2R1 interacts with other proteins such as CYP27B1 which further converts 25-hydroxyvitamin D to its active form.

CYP2R1's function deficiencies can directly relate to bone diseases like rickets or osteomalacia caused by insufficient vitamin D activation leading to bone weakening. Additionally mutations in the CYP2R1 gene can result in vitamin D-dependent rickets type 1B (VDDR1B) a hereditary disorder. The protein also shows an association with CYP27B1 in these conditions highlighting its importance in bone mineralization functions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol) (PubMed : 12867411, PubMed : 15465040, PubMed : 18511070). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives (PubMed : 15465040, PubMed : 18511070). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 12867411, PubMed : 15465040, PubMed : 18511070).
See full target information Vitamin D 25-hydroxylase
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 12: PubMed33172201

2020

Vitamin D Metabolite Profile in Cholecalciferol- or Calcitriol-Supplemented Healthy and Mammary Gland Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Artur Anisiewicz,Konrad Kowalski,Joanna Banach,Natalia Łabędź,Martyna Stachowicz-Suhs,Aleksandra Piotrowska,Magdalena Milczarek,Dagmara Kłopotowska,Piotr Dzięgiel,Joanna Wietrzyk

Biochemical pharmacology 155:547-561 PubMed30028992

2018

Strategies and limitations associated with in vitro characterization of vitamin D receptor activators.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Bukuroshi,Hiroshi Saitoh,Lilia Magomedova,Carolyn L Cummins,Edwin C Chow,Albert P Li,K Sandy Pang

Laboratory investigation; a journal of technical m 94:608-22 PubMed24732451

2014

Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Bergadà,Judit Pallares,Arcidiacono Maria Vittoria,Anna Cardus,Maria Santacana,Joan Valls,Gonzalo Cao,Elvira Fernàndez,Xavier Dolcet,Adriana S Dusso,Xavier Matias-Guiu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com