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AB239217

Anti-CYP2R1 antibody

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(1 Publication)

Goat Polyclonal CYP2R1 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Vitamin D 25-hydroxylase aa 400-450.

View Alternative Names

Vitamin D 25-hydroxylase, Cytochrome P450 2R1, Cyp2r1

1 Images
Western blot - Anti-CYP2R1 antibody (AB239217)
  • WB

Supplier Data

Western blot - Anti-CYP2R1 antibody (AB239217)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-CYP2R1 antibody (ab239217) at 2 µg/mL

Lane 1:

Mouse testis tissue lysate in RIPA buffer at 35 µg

Lane 2:

Rat testis tissue lysate in RIPA buffer at 35 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Vitamin D 25-hydroxylase aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q6VVW9

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Alternative Products

Primary Antibodies

AB137634

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2R1 antibody (AB137634)

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Complementary Products

Primary Antibodies

AB206655

Anti-CYP27B1 antibody [EPR20271]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] (AB206655)

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Primary Antibodies

AB188453

Anti-Dnmt1 antibody [EPR18453]

RabMAb|Recombinant|KO Validated|20ul selling size

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Primary Antibodies

AB126785

Anti-CYP27A1 antibody [EPR7529]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (AB126785)

  • 5
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Primary Antibodies

AB188470

Anti-Dnmt3a antibody [EPR18455]

RabMAb|Recombinant|KO Validated|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody [EPR18455] (AB188470)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP2R1 also known as Cytochrome P450 2R1 functions as a microsomal enzyme playing a major role in the hydroxylation of vitamin D. It catalyzes the conversion of vitamin D to 25-hydroxyvitamin D a form needed for further activation to the biologically active version. The protein has a known molecular mass of about 55 kDa. CYP2R1 is expressed in the liver which is important for its function owing to the liver's role in metabolizing vitamin D.
Biological function summary

CYP2R1 participates in the metabolic pathway to synthesize the active form of vitamin D. Although not part of a larger complex its activity serves a critical function in maintaining calcium homeostasis and bone health by mediating vitamin D metabolism. This process enables the regulation of calcium and phosphate essential for proper bone formation and maintenance.

Pathways

CYP2R1 plays an integral role in the vitamin D signaling pathway which is important for its metabolic conversion. Another related pathway is the cytochrome P450 metabolic pathway where CYP enzymes collaborate in synthesis and degradation processes. Within these pathways CYP2R1 interacts with other proteins such as CYP27B1 which further converts 25-hydroxyvitamin D to its active form.

CYP2R1's function deficiencies can directly relate to bone diseases like rickets or osteomalacia caused by insufficient vitamin D activation leading to bone weakening. Additionally mutations in the CYP2R1 gene can result in vitamin D-dependent rickets type 1B (VDDR1B) a hereditary disorder. The protein also shows an association with CYP27B1 in these conditions highlighting its importance in bone mineralization functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in activation of vitamin D precursors (PubMed : 12867411, PubMed : 24019477). Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (By similarity) (PubMed : 12867411, PubMed : 24019477).
See full target information Vitamin D 25-hydroxylase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurotoxicity research 40:1057-1069 PubMed35699893

2022

Geniposide Alleviates Neuropathic Pain in CCI Rats by Inhibiting the EGFR/PI3K/AKT Pathway And Ca Channels.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhang,Qiao-Qiao Chen,Li Yao
View all publications
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Product promise

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