AB65128

Anti-CYP2U1 antibody

Name: Anti-CYP2U1 antibody (ab65128) | Abcam
Brand: Abcam Limited
SKU: AB65128
Price: 535 USD
Availability: InStock

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(3 Publications )

Rabbit Polyclonal CYP2U1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 2U1.

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CYP2U1 antibody (AB65128)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CYP2U1 antibody (AB65128)

ICC/IF image of ab65128 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65128, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-CYP2U1 antibody (AB65128)

All lanes:

Western blot - Anti-CYP2U1 antibody (ab65128) at 1/500 dilution

Lane 1:

HeLa cell extract at 5 µg

Lane 2:

LOVO cell extract at 5 µg

Lane 3:

LOVO cell extract at 5 µg with immunising peptide

Predicted band size: 62 kDa

Observed band size: 28 kDa,55 kDa,62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Cytochrome P450 2U1. The exact immunogen used to generate this antibody is proprietary information.

Q7Z449

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP2U1 also known as Cytochrome P450 2U1 is an enzyme belonging to the cytochrome P450 family which plays an essential role in hydroxylation reactions and fatty acid metabolism. The protein has a mass of approximately 57 kDa. CYP2U1 is expressed mainly in brain thymus and liver tissues where its enzymatic activity modulates various metabolic processes.

Biological function summary

CYP2U1 processes long-chain fatty acids into hydroxy-fatty acids which are important for various cellular functions. It does not function as part of a larger enzyme complex but operates independently to influence lipid metabolism and energy balance in cells. This activity of CYP2U1 impacts physiological processes such as inflammation and neuronal health.

Pathways

CYP2U1 participates in the arachidonic acid and linoleic acid metabolism pathways. These pathways are significant because they link to the biosynthesis of important signaling molecules that mediate inflammatory responses and cell communication. CYP2U1 interacts with related proteins like CYP2C19 and CYP2J2 which also contribute to fatty acid metabolism.

Mutations or dysfunctions in CYP2U1 have connections with hereditary spastic paraplegia (HSP) and a specific type of cerebellar ataxia. HSP mutations in CYP2U1 affect neuronal pathways critical for motor function. In cerebellar ataxia CYP2U1 dysfunction affects proteins like spastin which result in impaired motor coordination and balance. Understanding CYP2U1's role in these disorders offers insights into potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of arachidonic acid and its conjugates (PubMed : 14660610, PubMed : 24563460). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 14660610, PubMed : 24563460). Acts as an omega and omega-1 hydroxylase for arachidonic acid and possibly for other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes (PubMed : 14660610, PubMed : 24563460). May down-regulate the biological activities of N-arachidonoyl-serotonin, an endocannabinoid that has anti-nociceptive effects through inhibition of fatty acid amide hydrolase FAAH, TRPV1 receptor and T-type calcium channels. Catalyzes C-2 oxidation of the indole ring of N-arachidonoyl-serotonin forming a less active product 2-oxo-N-arachidonoyl-serotonin (PubMed : 24563460).

See full target information Cytochrome P450 2U1

Alternative Names

Cytochrome P450 2U1, Long-chain fatty acid omega-monooxygenase, CYP2U1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of internal medicine 289:709-725 PubMed33107650

2021

Pseudoxanthoma elasticum overlaps hereditary spastic paraplegia type 56.

Applications

Unspecified application

Species

Unspecified reactive species

A Legrand,C Pujol,C M Durand,A Mesnil,I Rubera,C Duranton,S Zuily,A B Sousa,M Renaud,J L Boucher,N Pietrancosta,S Adham,C Orssaud,C Marelli,C Casali,L Ziccardi,N Villain,C Ewenczyk,A Durr,C Mignot,G Stevanin,C Billon,M Hureaux,X Jeunemaitre,C Goizet,J Albuisson

The international journal of biochemistry & cell b 110:111-121 PubMed30858141

2019

Glutamate affects the CYP1B1- and CYP2U1-mediated hydroxylation of arachidonic acid metabolism via astrocytic mGlu5 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xuming Yu,Juan Wu,Mingbai Hu,Jinhua Wu,Quanfei Zhu,Zheqiong Yang,Xianfei Xie,Yu-Qi Feng,Jiang Yue

Human mutation 39:140-151 PubMed29034544

2017

CYP2U1 activity is altered by missense mutations in hereditary spastic paraplegia 56.

Applications

Unspecified application

Species

Unspecified reactive species

Christelle M Durand,Laura Dhers,Christelle Tesson,Alessandra Tessa,Laetitia Fouillen,Stéphanie Jacqueré,Laure Raymond,Isabelle Coupry,Giovanni Benard,Frédéric Darios,Khalid H El-Hachimi,Guja Astrea,François Rivier,Guillaume Banneau,Claire Pujol,Didier Lacombe,Alexandra Durr,Patrick J Babin,Filippo M Santorelli,Nicolas Pietrancosta,Jean-Luc Boucher,Daniel Mansuy,Giovanni Stevanin,Cyril Goizet

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CYP2U1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CYP2U1 antibody (AB65128)

Western blot - Anti-CYP2U1 antibody (AB65128)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (3)

Product promise