AB22724

Anti-Cyp3a1 antibody [P6]

Name: Anti-Cyp3a1 antibody [P6] (ab22724) | Abcam
Brand: Abcam Limited
SKU: AB22724
Price: 565 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(12 Publications )

Mouse Monoclonal Cyp3a1 antibody. Suitable for Flow Cyt, WB, ELISA and reacts with Human, Pig, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Cyp3a1.

1 Images

Flow Cytometry - Anti-Cyp3a1 antibody [P6] (AB22724)

Flow Cyt

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Flow Cytometry - Anti-Cyp3a1 antibody [P6] (AB22724)

Overlay histogram showing HepG2 cells stained with ab22724 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22724, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat, Pig

Applications

Flow Cyt, WB, ELISA

applications

Immunogen

Full Length Protein corresponding to Rat Cyp3a1. The exact immunogen used to generate this antibody is proprietary information.

P04800

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

See full target information Cyp3a1

Alternative Names

Cyp3a-1, Cyp3a1, Cytochrome P450 3A1, CYPIIIA1, Cytochrome P450-PCN1

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Publications (12)

Recent publications for all applications. Explore the full listand refine your search

Scientific reports 15:18530 PubMed40425598

2025

Effects of mallotus furetianus extract on CYP3A activities in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Hyo In Kim,Feng Cao,Shuang Li,Jinbong Park,Peiqiong Li

Chemical & pharmaceutical bulletin 71:334-341 PubMed36858593

2023

Anti-tumor Effect of Gambogenic Acid and Its Effect on CYP2C and CYP3A after Oral Administration.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Sun,Mengdi Pang,Xiaozhu Tang,Qianqian Xu,Daiyin Peng,Weidong Chen

Cell reports 39:110879 PubMed35649362

2022

MDM2 binds and ubiquitinates PARP1 to enhance DNA replication fork progression.

Applications

Unspecified application

Species

Unspecified reactive species

Celeste Giansanti,Valentina Manzini,Antje Dickmanns,Achim Dickmanns,Maria Dilia Palumbieri,Andrea Sanchi,Simon Maria Kienle,Sonja Rieth,Martin Scheffner,Massimo Lopes,Matthias Dobbelstein

Stem cells international 2019:5310202 PubMed31885614

2019

The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yuan,Chunyou Lai,Lingling Wei,Tianhang Feng,Qinyan Yang,Tianying Zhang,Tao Lan,Yutong Yao,Guangming Xiang,Xiaolun Huang

Molecular medicine reports 20:771-778 PubMed31180561

2019

Effect of copper nanoparticles on brain cytochrome P450 enzymes in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Wang,Huaqiao Tang,Min Xu,Jie Luo,Ling Zhao,Fei Shi,Gang Ye,Cheng Lv,Yinglun Li

World journal of gastroenterology 23:7519-7530 PubMed29204052

2017

Pregnane X receptor and constitutive androstane receptor modulate differently CYP3A-mediated metabolism in early- and late-stage cholestasis.

Applications

Species

Rat

Daniela Gabbia,Arianna Dalla Pozza,Laura Albertoni,Roberta Lazzari,Giorgia Zigiotto,Maria Carrara,Vincenzo Baldo,Tatjana Baldovin,Annarosa Floreani,Sara De Martin

Basic & clinical pharmacology & toxicology 120:146-151 PubMed27611991

2016

Comparison of Pharmacokinetics and Tissue Distribution Kinetics of Roxithromycin and Expression of CYP 3A1 between Pregnant Mice and Foetuses.

Applications

Species

Mouse

Yi Qin,Weizhe Xu,Lanjian Mo,Xiaorong Li,Beikang Ge,Jie Xiong,Lifang Gao,Pinxiang Xu,Ming Xue

Drug metabolism and disposition: the biological fa 44:1524-35 PubMed27342868

2016

Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin C Y Chow,Jason Z Ya Wang,Holly P Quach,Hui Tang,David C Evans,Albert P Li,Jose Silva,K Sandy Pang

Journal of hepatology 63:1077-85 PubMed26144659

2015

Expression and functionality of Toll- and RIG-like receptors in HepaRG cells.

Applications

Unspecified application

Species

Unspecified reactive species

Souphalone Luangsay,Malika Ait-Goughoulte,Maud Michelet,Océane Floriot,Marc Bonnin,Marion Gruffaz,Michel Rivoire,Simon Fletcher,Hassan Javanbakht,Julie Lucifora,Fabien Zoulim,David Durantel

Toxicological sciences : an official journal of the Society of Toxicology 137:350-70 PubMed24235770

2013

Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies.

Applications

Species

Human

Philip M E Probert,Git W Chung,Simon J Cockell,Loranne Agius,Pasquale Mosesso,Steven A White,Fiona Oakley,Colin D A Brown,Matthew C Wright

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyp3a1 antibody [P6]

Flow Cytometry - Anti-Cyp3a1 antibody [P6] (AB22724)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (12)

Product promise