AB155029

Anti-CYP3A43 antibody

Name: Anti-CYP3A43 antibody (ab155029) | Abcam
Brand: Abcam Limited
SKU: AB155029

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(5 Publications)

Rabbit Polyclonal CYP3A43 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYP3A43 aa 1-250.

View Alternative Names

Cytochrome P450 3A43, CYP3A43

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A43 antibody (AB155029)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A43 antibody (AB155029)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling CYP3A43 using ab155029 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CYP3A43 antibody (AB155029)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CYP3A43 antibody (AB155029)

Immunofluorescence analysis of methanol fixed HeLa cells, labeling CYP3A43 using ab155029 at 1/200 dilution. Lower image costained with Hoechst 33342.

Unknown

Western blot - Anti-CYP3A43 antibody (AB155029)

10% SDS PAGE

All lanes:

Western blot - Anti-CYP3A43 antibody (ab155029) at 1/2000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CYP3A43 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9HB55

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP3A43 is an enzyme belonging to the cytochrome P450 superfamily also known by the alternate name Cytochrome P450 3A43. This enzyme has a molecular mass of approximately 57 kDa and features prominently in the metabolism of xenobiotics and drugs. CYP3A43 is mainly expressed in liver and small intestine tissues where it resides in the endoplasmic reticulum. It functions by oxidizing small foreign organic molecules such as toxins or drugs aiding in their clearance from the body.

Biological function summary

CYP3A43 plays significant roles in drug metabolism and synthesis of cholesterol steroids and other lipids. It does not appear to be part of a larger enzyme complex functioning independently within cells. Its activity is essential in transforming various substances into metabolites some of which are inactive while others may remain active. The enzyme is important for modulating the pharmacokinetics of drugs which includes absorption distribution metabolism and excretion of pharmaceutical compounds.

Pathways

CYP3A43 is involved in the xenobiotic metabolism pathway alongside other enzymes like CYP3A4 and CYP3A5. This pathway facilitates the detoxification of potentially harmful compounds. Additionally CYP3A43 participates in the metabolism of endogenous substrates contributing to the homeostasis of steroid hormones. Within these pathways it interacts with other cytochrome P450 enzymes sharing substantial substrate overlaps and sometimes competing with CYP3A4 which significantly influences the effects and side effects of drugs.

CYP3A43 has connections to alterations in drug metabolism that could lead to adverse drug reactions or ineffective therapy. Its variability in expression and activity can impact conditions such as cancer where drug metabolism is important for effective chemotherapy. Moreover the enzyme influences steroid hormone balance which may relate to disorders like hypertension or endocrine-related cancers. In these contexts CYP3A43 and related enzymes like CYP3A4 are pivotal in individual patient responses to drug treatments driving the need for personalized medicine approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits low testosterone 6-beta-hydroxylase activity.

See full target information CYP3A43

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 118:109267 PubMed31387005

2019

MicroRNA-519c-3p promotes tumor growth and metastasis of hepatocellular carcinoma by targeting BTG3.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Huanye Mo,Yezhen Jiang,Yufeng Wang,Liankang Sun,Bowen Yao,Tianxiang Chen,Runkun Liu,Qing Li,Qingguang Liu,Guozhi Yin

OncoTargets and therapy 11:7733-7743 PubMed30464522

2018

MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Du Meng,Ming Lei,Yaxuan Han,Dongli Zhao,Xiaozhi Zhang,Yunyi Yang,Rui Liu

OncoTargets and therapy 11:5885-5894 PubMed30271172

2018

MicroRNA-140-3p enhances the sensitivity of hepatocellular carcinoma cells to sorafenib by targeting pregnenolone X receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Li,Jing Zhao,Huan Wang,Xiaohan Li,Aixia Liu,Qin Qin,Boan Li

Oncogene 37:3216-3228 PubMed29511346

2018

Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Vikash Reebye,Kai-Wen Huang,Vivian Lin,Sheba Jarvis,Pedro Cutilas,Stephanie Dorman,Simona Ciriello,Pinelopi Andrikakou,Jon Voutila,Pal Saetrom,Paul J Mintz,Isabella Reccia,John J Rossi,Hans Huber,Robert Habib,Nikos Kostomitsopoulos,David C Blakey,Nagy A Habib

Toxicology research 5:278-290 PubMed30090344

2015

Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system.

Applications

Unspecified application

Species

Unspecified reactive species

M Richter,E A Fairhall,S A Hoffmann,S Tröbs,F Knöspel,P M E Probert,F Oakley,A Stroux,M C Wright,K Zeilinger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYP3A43 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A43 antibody (AB155029)

Immunocytochemistry/ Immunofluorescence - Anti-CYP3A43 antibody (AB155029)

Western blot - Anti-CYP3A43 antibody (AB155029)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise