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Rabbit Recombinant Monoclonal CYP3A5 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 8 publications.

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Images

Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (AB108624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (AB108624), expandable thumbnail
  • Western blot - Anti-CYP3A5 antibody [EPR4396] (AB108624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (AB108624), expandable thumbnail
  • Western blot - Anti-CYP3A5 antibody [EPR4396] (AB108624), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

For unpurified use at 1/20.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/400.

Tested
Tested

Species

Human

Dilution info

1/250

Notes

For unpurified use at 1/80.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins (PubMed:2732228, PubMed:10681376, PubMed:11093772, PubMed:12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:12865317, PubMed:2732228, PubMed:10681376, PubMed:11093772). Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed:12865317). Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione (PubMed:2732228). Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed:10681376). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed:11093772). Also involved in the oxidative metabolism of xenobiotics, including calcium channel blocking drug nifedipine and immunosuppressive drug cyclosporine (PubMed:2732228).

Alternative names

Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, CYP3A5

Recommended products

Rabbit Recombinant Monoclonal CYP3A5 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 8 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4396

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CYP3A5 also known as cytochrome P450 3A5 is an enzyme involved in drug metabolism. It is part of the cytochrome P450 superfamily. The enzyme has a molecular weight of approximately 57 kDa. Scientists have identified CYP3A5 as an important player in the oxidative metabolism of various xenobiotics and endogenous compounds. It is predominantly expressed in the liver but is also found in the kidney and intestines. Variability in CYP3A5 expression across individuals can significantly impact drug clearance rates and pharmacokinetics.

Biological function summary

CYP3A5 plays a significant role in the metabolism of many drugs and steroid hormones. This ability stems from its function as a monooxygenase in the metabolism of both exogenous and endogenous substrates. CYP3A5 is not necessarily part of a larger enzyme complex but it often acts in concert with other CYP3A enzymes within the liver. Variability in gene expression can result in different metabolizing capacities among individuals which scientists often relate to genetic polymorphisms that affect the CYP3A5*3 allele.

Pathways

CYP3A5 is integral to the steroid hormone biosynthesis and drug metabolism pathways. It interacts closely with CYP3A4 frequently working together to metabolize substrates such as cortisol and testosterone. The pathway involving drug metabolism highlights the importance of these enzymes in the detoxification and clearance of pharmaceuticals which may influence drug efficacy and safety in patients. Through its involvement in steroid hormone biosynthesis CYP3A5 coordinates with other enzymes to regulate the levels of active hormones contributing to hormonal balance.

Associated diseases and disorders

Scientists have linked variations in CYP3A5 expression and activity to hypertension and cancer. The role of CYP3A5 in hypertension is of particular interest due to its involvement in the metabolism of cortisol a hormone influencing blood pressure. Additionally altered CYP3A5 activity can affect the activation and clearance of chemotherapeutic agents impacting treatment outcomes in cancer. Researchers have noted that CYP3A5 shares functional relationships with proteins such as P-glycoprotein which plays roles in drug transport and resistance further connecting it to treatment efficacy and safety in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

  • Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    ab108624 (unpurified) at 1/20 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Predicted band size: 57 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/400 dilution

    All lanes: Human fetal liver tissue lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 57 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with unpurified ab108624 at 1/80. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution

    All lanes: Human fetal liver tissue lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 57 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human kidney tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    ab108624 (purified) at 1/50 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Predicted band size: 57 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    All lanes: Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: Human fetal colon tissue lysate at 10 µg

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human breast tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with purified ab108624 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

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