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AB56076

Anti-CYP3A5 antibody [F18 P3 B6]

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(1 Review)

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(1 Publication)

Mouse Monoclonal CYP3A5 antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 3A5.

View Alternative Names

Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, CYP3A5

3 Images
Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)
  • WB

Unknown

Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)

All lanes:

Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/mL

Lane 1:

CYP3A4 recombinant CYP450 protein

Lane 2:

CYP3A5 recombinant CYP450 protein

Lane 3:

CYP3A7 recombinant CYP450 protein

Predicted band size: 57 kDa

Observed band size: 57 kDa

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Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)
  • WB

Unknown

Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)

Lane 1:

Molecular weight markers

Lanes 2 - 10:

Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/mL

Lane 2:

CYP1A1 recombinant protein

Lane 3:

CYP2C18 recombinant protein

Lane 4:

CYP2C19 recombinant protein

Lane 5:

CYP2D6 recombinant protein

Lane 6:

CYP4F2 recombinant protein

Lane 7:

CYP4F3 recombinant protein

Lane 8:

CYP3A5 recombinant protein

Lane 9:

CYP3A7 recombinant protein

Lane 10:

Negative control

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [F18 P3 B6] (AB56076)

Oesophageal squamous cell carcinoma tissue stained with ab56076 at 5 µg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F18 P3 B6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Cytochrome P450 3A5. The exact immunogen used to generate this antibody is proprietary information.

P20815

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP3A5 also known as cytochrome P450 3A5 is an enzyme involved in drug metabolism. It is part of the cytochrome P450 superfamily. The enzyme has a molecular weight of approximately 57 kDa. Scientists have identified CYP3A5 as an important player in the oxidative metabolism of various xenobiotics and endogenous compounds. It is predominantly expressed in the liver but is also found in the kidney and intestines. Variability in CYP3A5 expression across individuals can significantly impact drug clearance rates and pharmacokinetics.
Biological function summary

CYP3A5 plays a significant role in the metabolism of many drugs and steroid hormones. This ability stems from its function as a monooxygenase in the metabolism of both exogenous and endogenous substrates. CYP3A5 is not necessarily part of a larger enzyme complex but it often acts in concert with other CYP3A enzymes within the liver. Variability in gene expression can result in different metabolizing capacities among individuals which scientists often relate to genetic polymorphisms that affect the CYP3A5*3 allele.

Pathways

CYP3A5 is integral to the steroid hormone biosynthesis and drug metabolism pathways. It interacts closely with CYP3A4 frequently working together to metabolize substrates such as cortisol and testosterone. The pathway involving drug metabolism highlights the importance of these enzymes in the detoxification and clearance of pharmaceuticals which may influence drug efficacy and safety in patients. Through its involvement in steroid hormone biosynthesis CYP3A5 coordinates with other enzymes to regulate the levels of active hormones contributing to hormonal balance.

Scientists have linked variations in CYP3A5 expression and activity to hypertension and cancer. The role of CYP3A5 in hypertension is of particular interest due to its involvement in the metabolism of cortisol a hormone influencing blood pressure. Additionally altered CYP3A5 activity can affect the activation and clearance of chemotherapeutic agents impacting treatment outcomes in cancer. Researchers have noted that CYP3A5 shares functional relationships with proteins such as P-glycoprotein which plays roles in drug transport and resistance further connecting it to treatment efficacy and safety in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins (PubMed : 10681376, PubMed : 11093772, PubMed : 12865317, PubMed : 2732228). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed : 10681376, PubMed : 11093772, PubMed : 12865317, PubMed : 2732228). Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed : 12865317). Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione (PubMed : 2732228). Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed : 10681376). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed : 11093772). Also involved in the oxidative metabolism of xenobiotics, including calcium channel blocking drug nifedipine and immunosuppressive drug cyclosporine (PubMed : 2732228).
See full target information Cytochrome P450 3A5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Future medicinal chemistry 11:2891-2903 PubMed31702381

2019

A dual functional probe for assessing human CYP450 3A5 and 3A enzymes bioactivities.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yan,Yonglei Cui,Yue An,Jing Ning,Xinyu Zhao,Lei Feng,Xiaokui Huo,Chao Wang,Chuanzhu Lv,Xiaochi Ma,Xiangge Tian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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