Rabbit Polyclonal CYP3A7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP3A7 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYP3A7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P24462
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis (PubMed:11093772, PubMed:12865317, PubMed:14559847, PubMed:17178770, PubMed:9555064). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:11093772, PubMed:12865317, PubMed:14559847, PubMed:17178770, PubMed:9555064). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones (PubMed:17178770, PubMed:9555064). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position (PubMed:12865317, PubMed:14559847). Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development (PubMed:11093772). Also involved in the oxidative metabolism of xenobiotics including anticonvulsants (PubMed:9555064).
Alternative names
Cytochrome P450 3A7, CYPIIIA7, Cytochrome P450-HFLA, P450HLp2, CYP3A7
Recommended products
Rabbit Polyclonal CYP3A7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP3A7 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYP3A7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P24462
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CYP3A7 also known as cytochrome P450 3A7 is an enzyme weighing approximately 57 kDa and belongs to the cytochrome P450 superfamily. This enzyme primarily facilitates the oxidation of various substrates including steroids fatty acids and xenobiotics. CYP3A7 expression is largely found in fetal liver tissue but shows decreased levels after birth. As part of the cytochrome P450 family CYP3A7 plays an important role in metabolic processes that modify and clear compounds from the body.
- Biological function summary
CYP3A7 acts on endogenous and exogenous molecules influencing steroid metabolism and drug catabolism. This enzyme acts individually rather than as part of a larger enzyme complex. Its function contributes significantly to the metabolism of retinoic acid and other steroid hormones during fetal development. The enzyme's substrate specificity enables it to play a role in modulating biological availabilities of several substances during prenatal human development.
- Pathways
CYP3A7's enzymatic activity integrates into the retinoic acid metabolism and steroid hormone biosynthesis pathways. It's actively involved in processing compounds needed for proper fetal growth and development such as all-trans-retinoic acid. Its relatives like CYP3A4 and CYP3A5 perform similar roles in adults showing high homology and overlapping substrate ranges reflecting their functional association within these pathways.
- Associated diseases and disorders
Researchers link CYP3A7 to alterations in drug metabolism affecting pharmacokinetics and therapeutic outcomes. For instance the enzyme's atypical activity can influence glucocorticoid metabolism connecting it to disorders like congenital adrenal hyperplasia. CYP3A7's function can also interact with proteins such as CYP21A2 further impacting steroidogenesis-related diseases. Understanding variations in CYP3A7 activity contributes to insights into certain congenital disorders and can guide personalized approaches in drug therapy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A7 antibody (ab239020)
Paraffin-embedded human liver tissue stained for CYP3A7 with ab239020 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-CYP3A7 antibody (ab239020)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP3A7 using ab239020 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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