AB169764

Anti-CYP3A7 antibody [EPR10650(2)(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal CYP3A7 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Cytochrome P450 3A7, CYPIIIA7, Cytochrome P450-HFLA, P450HLp2, CYP3A7

1 Images

Unknown

Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] (AB169764)

All lanes:

Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] (ab169764) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa

false

Carrier free

Anti-CYP3A7 antibody [EPR10650(2)(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10650(2)(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5001", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP3A7 also known as cytochrome P450 3A7 is an enzyme weighing approximately 57 kDa and belongs to the cytochrome P450 superfamily. This enzyme primarily facilitates the oxidation of various substrates including steroids fatty acids and xenobiotics. CYP3A7 expression is largely found in fetal liver tissue but shows decreased levels after birth. As part of the cytochrome P450 family CYP3A7 plays an important role in metabolic processes that modify and clear compounds from the body.

Biological function summary

CYP3A7 acts on endogenous and exogenous molecules influencing steroid metabolism and drug catabolism. This enzyme acts individually rather than as part of a larger enzyme complex. Its function contributes significantly to the metabolism of retinoic acid and other steroid hormones during fetal development. The enzyme's substrate specificity enables it to play a role in modulating biological availabilities of several substances during prenatal human development.

Pathways

CYP3A7's enzymatic activity integrates into the retinoic acid metabolism and steroid hormone biosynthesis pathways. It's actively involved in processing compounds needed for proper fetal growth and development such as all-trans-retinoic acid. Its relatives like CYP3A4 and CYP3A5 perform similar roles in adults showing high homology and overlapping substrate ranges reflecting their functional association within these pathways.

Researchers link CYP3A7 to alterations in drug metabolism affecting pharmacokinetics and therapeutic outcomes. For instance the enzyme's atypical activity can influence glucocorticoid metabolism connecting it to disorders like congenital adrenal hyperplasia. CYP3A7's function can also interact with proteins such as CYP21A2 further impacting steroidogenesis-related diseases. Understanding variations in CYP3A7 activity contributes to insights into certain congenital disorders and can guide personalized approaches in drug therapy.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis (PubMed : 11093772, PubMed : 12865317, PubMed : 14559847, PubMed : 17178770, PubMed : 9555064). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 11093772, PubMed : 12865317, PubMed : 14559847, PubMed : 17178770, PubMed : 9555064). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones (PubMed : 17178770, PubMed : 9555064). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position (PubMed : 12865317, PubMed : 14559847). Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development (PubMed : 11093772). Also involved in the oxidative metabolism of xenobiotics including anticonvulsants (PubMed : 9555064).

See full target information CYP3A7

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38786098

2024

PI 3-Kinase and the Histone Methyl-Transferase KMT2D Collaborate to Induce Arp2/3-Dependent Migration of Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Karina D Rysenkova,Julia Gaboriaud,Artem I Fokin,Raphaëlle Toubiana,Alexandre Bense,Camil Mirdass,Mélissa Jin,Minh Chau N Ho,Elizabeth Glading,Sophie Vacher,Laura Courtois,Ivan Bièche,Alexis M Gautreau

Current protocols 4:e1003 PubMed38483112

2024

Use of Lentivirus-Based Method for Establishing TK6 Human Cell Lines Expressing Cytochrome P450 and its Applications in Genotoxicity Testing.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Li,Si Chen,Xiaobo He,Qiangen Wu,Lei Guo,Nan Mei

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYP3A7 antibody [EPR10650(2)(B)]

Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] (AB169764)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise