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AB249511

Anti-CYP3A7 antibody [EPR10650(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CYP3A7 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cytochrome P450 3A7, CYPIIIA7, Cytochrome P450-HFLA, P450HLp2, CYP3A7

1 Images
Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] - BSA and Azide free (AB249511)
  • WB

Unknown

Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] - BSA and Azide free (AB249511)

This data was developed using ab169764, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CYP3A7 antibody [EPR10650(2)(B)] (<a href='/en-us/products/primary-antibodies/cyp3a7-antibody-epr106502b-ab169764'>ab169764</a>) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10650(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249511 is the carrier-free version of ab169764.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP3A7 also known as cytochrome P450 3A7 is an enzyme weighing approximately 57 kDa and belongs to the cytochrome P450 superfamily. This enzyme primarily facilitates the oxidation of various substrates including steroids fatty acids and xenobiotics. CYP3A7 expression is largely found in fetal liver tissue but shows decreased levels after birth. As part of the cytochrome P450 family CYP3A7 plays an important role in metabolic processes that modify and clear compounds from the body.
Biological function summary

CYP3A7 acts on endogenous and exogenous molecules influencing steroid metabolism and drug catabolism. This enzyme acts individually rather than as part of a larger enzyme complex. Its function contributes significantly to the metabolism of retinoic acid and other steroid hormones during fetal development. The enzyme's substrate specificity enables it to play a role in modulating biological availabilities of several substances during prenatal human development.

Pathways

CYP3A7's enzymatic activity integrates into the retinoic acid metabolism and steroid hormone biosynthesis pathways. It's actively involved in processing compounds needed for proper fetal growth and development such as all-trans-retinoic acid. Its relatives like CYP3A4 and CYP3A5 perform similar roles in adults showing high homology and overlapping substrate ranges reflecting their functional association within these pathways.

Researchers link CYP3A7 to alterations in drug metabolism affecting pharmacokinetics and therapeutic outcomes. For instance the enzyme's atypical activity can influence glucocorticoid metabolism connecting it to disorders like congenital adrenal hyperplasia. CYP3A7's function can also interact with proteins such as CYP21A2 further impacting steroidogenesis-related diseases. Understanding variations in CYP3A7 activity contributes to insights into certain congenital disorders and can guide personalized approaches in drug therapy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis (PubMed : 11093772, PubMed : 12865317, PubMed : 14559847, PubMed : 17178770, PubMed : 9555064). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 11093772, PubMed : 12865317, PubMed : 14559847, PubMed : 17178770, PubMed : 9555064). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones (PubMed : 17178770, PubMed : 9555064). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position (PubMed : 12865317, PubMed : 14559847). Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development (PubMed : 11093772). Also involved in the oxidative metabolism of xenobiotics including anticonvulsants (PubMed : 9555064).
See full target information CYP3A7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

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