AB244241

Anti-CYP46A1 antibody [1A7]

BOND RX™ Validated
Recombinant
What is this?

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(5 Publications)

Mouse Recombinant Monoclonal CYP46A1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

CYP46, CYP46A1, Cholesterol 24-hydroxylase, CH24H, Cholesterol 24-monooxygenase, Cholesterol 24S-hydroxylase, Cytochrome P450 46A1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46A1 antibody [1A7] (AB244241)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46A1 antibody [1A7] (AB244241)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CYP46 with ab244241 at 8.6μg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum. The section was incubated with ab244241 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-CYP46A1 antibody [1A7] (AB244241)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CYP46A1 antibody [1A7] (ab244241) at 0.43 µg/mL

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 56 kDa

false

Carrier free

Anti-CYP46A1 antibody [1A7] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A7

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.43 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "8.6 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.43 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "8.6 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.43 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "8.6 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP46A1 also known as cholesterol 24-hydroxylase is a member of the cytochrome P450 superfamily. It has a molecular mass of approximately 56 kDa. CYP46A1 is expressed mainly in the brain specifically within neurons that are active in cholesterol metabolism. This enzyme localizes in the endoplasmic reticulum and is responsible for converting cholesterol into 24S-hydroxycholesterol a more soluble form that can cross the blood-brain barrier.

Biological function summary

The conversion of cholesterol to 24S-hydroxycholesterol by CYP46A1 helps maintain cholesterol homeostasis in the central nervous system. This process is not part of a large complex but it plays an important role in the clearance of excess cholesterol from the brain. The removal of cholesterol metabolites is essential for normal brain function and neuronal health.

Pathways

CYP46A1's activity fits into the cholesterol catabolism pathway to regulate cholesterol levels specifically in the brain. The enzyme interacts with pathways that include cholesterol turnover and removal from the central nervous system. In this context CYP46A1 is related to APOE a protein involved in lipid metabolism and transport contributing to the balance of cholesterol and lipids in the brain.

Alterations in CYP46A1 have associations with Alzheimer’s disease and Huntington’s disease. In Alzheimer’s disease abnormal cholesterol metabolism involving CYP46A1 may result in the formation of amyloid plaques as the enzyme is linked to APP which is important in the pathology of Alzheimer's. In Huntington’s disease disruption of cholesterol homeostasis by dysregulation of CYP46A1 might aggravate neuronal degeneration suggesting a potential therapeutic target for neuroprotection.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed : 10377398, PubMed : 14640697, PubMed : 18621681, PubMed : 25017465). By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity). Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (PubMed : 12077124, PubMed : 14640697, PubMed : 28190002). Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (PubMed : 25017465). May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (PubMed : 20667828).

See full target information CYP46A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112992 PubMed40687785

2025

Differential substrate degradation by super-degron: EGFP in wild-type mouse cells, PD-1 requires CRBN humanization.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Naruse,Ojiro Ishibashi,Masatoshi Ohgushi,Hirohiko Imai,Tomoko Matsuzaki,Xuchi Pan,Tatsuhiko Miyazaki,Yuka Shidahara,Yu Shirakawa,Fumihiro Sugiyama,Masahide Asano

International journal of molecular sciences 23: PubMed35457188

2022

Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Shanshan Cui,Ling Hao,Wen Liu,Lijing Wang,Mengwei Ju,Wenjing Feng,Rong Xiao

International journal of molecular sciences 22: PubMed34299071

2021

Liver Bile Acid Changes in Mouse Models of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Harpreet Kaur,Drew Seeger,Svetlana Golovko,Mikhail Golovko,Colin Kelly Combs

PloS one 12:e0172552 PubMed28231263

2017

Uniformity under in vitro conditions: Changes in the phenotype of cancer cell lines derived from different medulloblastoma subgroups.

Applications

Unspecified application

Species

Unspecified reactive species

Petr Chlapek,Karel Zitterbart,Leos Kren,Lenka Filipova,Jaroslav Sterba,Renata Veselska

The Journal of comparative neurology 507:1676-93 PubMed18241055

2008

Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Denise M O Ramirez,Stefan Andersson,David W Russell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CYP46A1 antibody [1A7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46A1 antibody [1A7] (AB244241)

Western blot - Anti-CYP46A1 antibody [1A7] (AB244241)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise