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AB110012

Anti-CYP4B1 antibody

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(1 Publication)

Rabbit Polyclonal CYP4B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 4B1.

View Alternative Names

Cytochrome P450 4B1, CYPIVB1, Cytochrome P450-HP, CYP4B1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP4B1 antibody (AB110012)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP4B1 antibody (AB110012)

Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue, using ab110012 at 1/50. The picture on the right is treated with the synthesized peptide.

Western blot - Anti-CYP4B1 antibody (AB110012)
  • WB

Unknown

Western blot - Anti-CYP4B1 antibody (AB110012)

All lanes:

Western blot - Anti-CYP4B1 antibody (ab110012) at 1/500 dilution

All lanes:

extracts from COLO cells (5-30 ug total protein) with no immunizing peptide

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Cytochrome P450 4B1. The exact immunogen used to generate this antibody is proprietary information.

P13584

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYP4B1 also known as Cytochrome P450 4B1 is an enzyme part of the Cytochrome P450 family which plays a significant role in metabolizing xenobiotics and endogenous compounds. It has a molecular mass of approximately 58 kDa. This enzyme is found in various tissues with high expression levels in the lung and liver tissues. The CYP4B1 catalyzes reactions involved in the oxidation of several substrates making it important for metabolic processes.
Biological function summary

The enzyme acts mainly in the oxidation of fatty acids and other lipophilic compounds. It contributes to the activation and detoxification of xenobiotic substances. Although not a part of any large protein complex CYP4B1 works closely with its cofactors like NADPH-cytochrome P450 reductase to achieve its function. This interaction is essential for its role in the metabolic processes that maintain cellular homeostasis.

Pathways

CYP4B1 is involved in both the arachidonic acid metabolism and the eicosanoid synthesis pathways. These pathways are significant for producing signaling molecules that regulate inflammation and other physiological functions. CYP4B1 interacts with proteins such as ALOX5 (arachidonate 5-lipoxygenase) within these pathways contributing to the complex network that controls inflammatory responses and other related bioactivities.

Investigations have connected CYP4B1 to carcinogenesis and inflammatory lung diseases. Abnormal CYP4B1 function is often associated with cancer due to its role in metabolic activation of certain procarcinogens. It also shows a relationship with lung inflammation where its activity may influence disease progression. During these conditions alterations in CYP4B1 activity can modify the bioavailability of signaling molecules linking it to other proteins like COX-2 (Cyclooxygenase-2) that are central in inflammation-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
See full target information Cytochrome P450 4B1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 11: PubMed33207796

2020

Identification of Milk Fat Metabolism-Related Pathways of the Bovine Mammary Gland during Mid and Late Lactation and Functional Verification of the Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Yongliang Fan,Ziyin Han,Xubin Lu,Huimin Zhang,Abdelaziz Adam Idriss Arbab,Juan J Loor,Yi Yang,Zhangping Yang
View all publications
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Product promise

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