Rabbit Polyclonal CYP4B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 4B1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Cytochrome P450 4B1. The exact immunogen used to generate this antibody is proprietary information. Database link P13584
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Target data
Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Alternative names
Cytochrome P450 4B1, CYPIVB1, Cytochrome P450-HP, CYP4B1
Recommended products
Rabbit Polyclonal CYP4B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 4B1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Cytochrome P450 4B1. The exact immunogen used to generate this antibody is proprietary information. Database link P13584
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CYP4B1 also known as Cytochrome P450 4B1 is an enzyme part of the Cytochrome P450 family which plays a significant role in metabolizing xenobiotics and endogenous compounds. It has a molecular mass of approximately 58 kDa. This enzyme is found in various tissues with high expression levels in the lung and liver tissues. The CYP4B1 catalyzes reactions involved in the oxidation of several substrates making it important for metabolic processes.
- Biological function summary
The enzyme acts mainly in the oxidation of fatty acids and other lipophilic compounds. It contributes to the activation and detoxification of xenobiotic substances. Although not a part of any large protein complex CYP4B1 works closely with its cofactors like NADPH-cytochrome P450 reductase to achieve its function. This interaction is essential for its role in the metabolic processes that maintain cellular homeostasis.
- Pathways
CYP4B1 is involved in both the arachidonic acid metabolism and the eicosanoid synthesis pathways. These pathways are significant for producing signaling molecules that regulate inflammation and other physiological functions. CYP4B1 interacts with proteins such as ALOX5 (arachidonate 5-lipoxygenase) within these pathways contributing to the complex network that controls inflammatory responses and other related bioactivities.
- Associated diseases and disorders
Investigations have connected CYP4B1 to carcinogenesis and inflammatory lung diseases. Abnormal CYP4B1 function is often associated with cancer due to its role in metabolic activation of certain procarcinogens. It also shows a relationship with lung inflammation where its activity may influence disease progression. During these conditions alterations in CYP4B1 activity can modify the bioavailability of signaling molecules linking it to other proteins like COX-2 (Cyclooxygenase-2) that are central in inflammation-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP4B1 antibody (ab110012)
Immunohistochemistry analysis of paraffin embedded human lung carcinoma tissue, using ab110012 at 1/50. The picture on the right is treated with the synthesized peptide.
Western blot - Anti-CYP4B1 antibody (ab110012)
All lanes: Western blot - Anti-CYP4B1 antibody (ab110012) at 1/500 dilution
All lanes: extracts from COLO cells (5-30 ug total protein) with no immunizing peptide
Predicted band size: 59 kDa
Downloads
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