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AB230709

Anti-CYP4F2 antibody - N-terminal

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(3 Publications)

Rabbit Polyclonal CYP4F2 antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome P450 4F2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Cytochrome P450 4F2, 20-hydroxyeicosatetraenoic acid synthase, Arachidonic acid omega-hydroxylase, CYPIVF2, Cytochrome P450-LTB-omega, Docosahexaenoic acid omega-hydroxylase, Leukotriene-B(4) 20-monooxygenase 1, Leukotriene-B(4) omega-hydroxylase 1, Phylloquinone omega-hydroxylase CYP4F2, 20-HETE synthase, CYP4F2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP4F2 antibody - N-terminal (AB230709)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP4F2 antibody - N-terminal (AB230709)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for CYP4F2 using ab230709 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CYP4F2 antibody - N-terminal (AB230709)
  • WB

Supplier Data

Western blot - Anti-CYP4F2 antibody - N-terminal (AB230709)

All lanes:

Western blot - Anti-CYP4F2 antibody - N-terminal (ab230709) at 1/500 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Cytochrome P450 4F2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P78329

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (AB179800)

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Primary Antibodies

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Anti-CYP1B1 antibody [EPR14972] - C-terminal

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (AB185954)

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Primary Antibodies

AB230788

Anti-TR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR1 antibody (AB230788)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP4F2 also known as cytochrome P450 4F2 is an enzyme that catalyzes the metabolism of certain fatty acids and drugs. It possesses a mass of approximately 60 kDa and is primarily expressed in the liver and kidney with noticeable presence in other tissues like the small intestine. This protein plays a role in omega-hydroxylation of fatty acids including arachidonic acid essential for producing signaling molecules called eicosanoids.
Biological function summary

The omega-hydroxylation by CYP4F2 converts fatty acids into hydroxylated derivatives which regulate inflammation and blood pressure. CYP4F2 is not part of a larger complex but interacts with molecular components like heme as a cofactor to carry out its enzymatic functions. Its activity contributes significantly to the body's ability to process and eliminate certain medications and carcinogens.

Pathways

CYP4F2 plays integral roles in the arachidonic acid metabolism pathway and vitamin K-dependent biosynthesis. During these processes CYP4F2 participates alongside related enzymes like CYP2C9 and CYP3A4. In arachidonic acid metabolism it contributes to converting arachidonic acid into 20-hydroxyeicosatetraenoic acid (20-HETE) an active lipid mediator affecting vascular tone.

CYP4F2 has been linked to hypertension and bleeding disorders. Variants in the CYP4F2 gene can affect the enzyme’s ability to metabolize agents involved in blood pressure regulation and warfarin metabolism influencing the risk and management of such conditions. The enzyme’s interaction with cytochrome P450 enzymes including CYP2C9 associated with warfarin metabolism highlights its relevance in pharmacogenetics and personalized medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, eicosanoids and vitamins (PubMed : 10660572, PubMed : 10833273, PubMed : 11997390, PubMed : 17341693, PubMed : 18574070, PubMed : 18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of long- and very long-chain fatty acids. Displays high omega-hydroxylase activity toward polyunsaturated fatty acids (PUFAs) (PubMed : 18577768). Participates in the conversion of arachidonic acid to omega-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed : 10660572, PubMed : 17341693, PubMed : 18574070). Plays a role in the oxidative inactivation of eicosanoids, including both pro-inflammatory and anti-inflammatory mediators such as leukotriene B4 (LTB4), lipoxin A4 (LXA4), and several HETEs (PubMed : 10660572, PubMed : 10833273, PubMed : 17341693, PubMed : 18574070, PubMed : 18577768, PubMed : 8026587, PubMed : 9799565). Catalyzes omega-hydroxylation of 3-hydroxy fatty acids (PubMed : 18065749). Converts monoepoxides of linoleic acid leukotoxin and isoleukotoxin to omega-hydroxylated metabolites (PubMed : 15145985). Contributes to the degradation of very long-chain fatty acids (VLCFAs) by catalyzing successive omega-oxidations and chain shortening (PubMed : 16547005, PubMed : 18182499). Plays an important role in vitamin metabolism by chain shortening. Catalyzes omega-hydroxylation of the phytyl chain of tocopherols (forms of vitamin E), with preference for gamma-tocopherols over alpha-tocopherols, thus promoting retention of alpha-tocopherols in tissues (PubMed : 11997390). Omega-hydroxylates and inactivates phylloquinone (vitamin K1), and menaquinone-4 (MK-4, a form of vitamin K2), both acting as cofactors in blood coagulation (PubMed : 19297519, PubMed : 24138531).
See full target information Cytochrome P450 4F2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iMeta 4:e70037 PubMed40860437

2025

Maternal gut microbiota-derived daidzein prevents osteoporosis in female offspring following prenatal prednisone exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Ma,Hangyuan He,Kunpeng Wang,Juanjuan Guo,Liang Liu,Yuting Chen,Bin Li,Hao Xiao,Xufeng Li,Xiaoqian Lu,Tingting Wang,Yinxian Wen,Hui Wang,Liaobin Chen

Frontiers in pharmacology 15:1438567 PubMed39188949

2024

11-Hydroxyeicosatetraenoics induces cellular hypertrophy in an enantioselective manner.

Applications

Unspecified application

Species

Unspecified reactive species

Sara A Helal,Ahmed A El-Sherbeni,Ayman O S El-Kadi

Biochemistry and biophysics reports 38:101706 PubMed38646426

2024

Targeting estrogen mediated CYP4F2/CYP4F11-20-HETE metabolic disorder decelerates tumorigenesis in ER+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Yang,Yin Li,Xiao Han,Tianjiao Li,Ding Li,Qiao Liu,Lizhong Yan,Fei Li,Xiaolin Pei,Ya Feng,Zhoujun Lin,Zhenkun Fu,Changjun Wang,Qiang Sun,Chenggang Li
View all publications
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