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AB69392

Anti-CYP4V2 antibody

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(3 Publications)

Mouse Polyclonal CYP4V2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Cytochrome P450 4V2.

View Alternative Names

Cytochrome P450 4V2, Docosahexaenoic acid omega-hydroxylase CYP4V2, Long-chain fatty acid omega-monooxygenase, CYP4V2

1 Images
Western blot - Anti-CYP4V2 antibody (AB69392)
  • WB

Unknown

Western blot - Anti-CYP4V2 antibody (AB69392)

All lanes:

Western blot - Anti-CYP4V2 antibody (ab69392) at 1/500 dilution

All lanes:

human liver lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Cytochrome P450 4V2.

Q6ZWL3

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Assay Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP4V2 also known as cytochrome P450 family 4 subfamily V member 2 is an enzyme with a molecular mass of approximately 61 kDa. This protein functions as part of the cytochrome P450 superfamily specifically catalyzing the oxidation of fatty acids and steroid metabolism. CYP4V2 is expressed in various tissues especially prominent in the retina. It helps in the biochemical processes involving lipid metabolism important for maintaining cellular homeostasis.
Biological function summary

CYP4V2 plays a significant role in the metabolism of long-chain fatty acids. It does not form part of a larger complex but functions independently to influence the metabolism pathways critical for maintaining normal visual function. Its activity is essential for processing specific fatty acids that accumulate due to metabolic dysfunction which can lead to cellular damage if not properly managed.

Pathways

The enzyme participates notably in the omega-hydroxylation pathway important for lipid processing. The long-chain fatty acids are metabolized through reactions facilitated by CYP4V2 which ensures the regulation of lipid synthesis and degradation. Alongside other members of the cytochrome P450 family such as CYP4A11 CYP4V2 maintains systemic balance through these processes.

CYP4V2 mutation is closely linked to Bietti's crystalline dystrophy a retinal degenerative disease causing vision impairment. This connection highlights its critical function in retinal lipid metabolism. The disruption in the normal function of CYP4V2 impacts the homeostatic mechanisms similar disorders may synergistically involve proteins such as ABCA4 contributing to the pathogenesis of retinal deterioration. Understanding these interactions is important for developing targeted disease-modifying therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in fatty acid metabolism in the eye. Catalyzes the omega-hydroxylation of polyunsaturated fatty acids (PUFAs) docosahexaenoate (DHA) and its precursor eicosapentaenoate (EPA), and may contribute to the homeostasis of these retinal PUFAs (PubMed : 22772592). Omega hydroxylates saturated fatty acids such as laurate, myristate and palmitate, the catalytic efficiency decreasing in the following order : myristate > laurate > palmitate (C14>C12>C16) (PubMed : 19661213). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
See full target information Cytochrome P450 4V2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:359 PubMed38992691

2024

Uncovering the role of ferroptosis in Bietti crystalline dystrophy and potential therapeutic strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Shen,Qianjie Yang,Kuangqi Chen,Huiling Ma,Xiawei Wang,Jianping Tong,Ye Shen,Hongguang Cui

Scientific reports 12:9525 PubMed35680963

2022

AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Hui Wang,Grace E Lidgerwood,Maciej Daniszewski,Monica L Hu,Georgina E Roberts,Raymond C B Wong,Sandy S C Hung,Michelle E McClements,Alex W Hewitt,Alice Pébay,Doron G Hickey,Thomas L Edwards

The Journal of biological chemistry 289:11132-42 PubMed24573683

2014

Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Tashima,Pamela Stanley
View all publications
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Product promise

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