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AB210792

Anti-CYP51A1/CYP51 antibody

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(4 Publications)

Rabbit Polyclonal CYP51A1/CYP51 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYP51A1 aa 300 to C-terminus.

View Alternative Names

CYP51, CYP51A1, Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P450LI, Sterol 14-alpha demethylase, Cytochrome P45014DM

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CYP51A1/CYP51 antibody (AB210792)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYP51A1/CYP51 antibody (AB210792)

Immunofluorescent analysis of MCF7 cells labeling CYP51A1/CYP51 with ab210792 at 1/20 dilution. Blue : DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP51A1/CYP51 antibody (AB210792)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP51A1/CYP51 antibody (AB210792)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CYP51A1/CYP51 with ab210792 at 1/100 dilution.

Western blot - Anti-CYP51A1/CYP51 antibody (AB210792)
  • WB

Supplier Data

Western blot - Anti-CYP51A1/CYP51 antibody (AB210792)

All lanes:

Western blot - Anti-CYP51A1/CYP51 antibody (ab210792) at 1/500 dilution

Lane 1:

Mouse liver extract

Lane 2:

Mouse kidney extract

Lane 3:

Rat liver extract

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CYP51A1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16850

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP51A1 also known as CYP51 is a cytochrome P450 enzyme. It plays an important mechanical role in cholesterol biosynthesis specifically catalyzing the demethylation of lanosterol. This enzyme has a molecular mass of approximately 55 kDa. CYP51A1 is expressed in multiple tissues including the liver testes and adrenal glands reflecting its widespread functional importance within the body.
Biological function summary

CYP51A1 is essential in the production of sterols such as cholesterol which are fundamental components of cell membranes. It does not operate alone; rather it forms part of an enzymatic complex involved in lipid homeostasis. By participating in this complex CYP51A1 influences cell membrane integrity fluidity and signaling.

Pathways

The activity of CYP51A1 integrates into the cholesterol biosynthesis pathway where it serves a critical role in the downstream conversion of lanosterol to cholesterol. Its action influences the pathway alongside other key enzymes like HMG-CoA reductase. Additionally the enzyme participates in broader lipid metabolic pathways linking with proteins such as squalene synthase that also contribute to sterol production.

CYP51A1's activity associates with certain lipid metabolism disorders including congenital adrenal hyperplasia and Smith-Lemli-Opitz syndrome. These conditions often involve disrupted cholesterol synthesis where CYP51A1’s malfunction can play a part. Moreover CYP51A1 connects to liver function through its interaction with proteins such as sterol regulatory element binding proteins (SREBPs) which regulate cholesterol homeostasis and can be associated with liver diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis (PubMed : 20149798, PubMed : 8619637, PubMed : 9559662). Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis (PubMed : 20149798, PubMed : 8619637, PubMed : 9559662). Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL (PubMed : 9559662).
See full target information CYP51A1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405240 PubMed39234807

2024

Spatial Transcriptomic Study Reveals Heterogeneous Metabolic Adaptation and a Role of Pericentral PPARα/CAR/Ces2a Axis During Fasting in Mouse Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Shiguan Wang,Bowen Xu,Jinyuan Liang,Yawei Feng,Penghu Han,Jing Shen,Xinying Li,Mengqi Zheng,Tingguo Zhang,Cuijuan Zhang,Ping Mi,Yi Zhang,Zhiping Liu,Shiyang Li,Detian Yuan

International journal of biological sciences 20:3590-3620 PubMed38993563

2024

Machine Learning-based Framework Develops a Tumor Thrombus Coagulation Signature in Multicenter Cohorts for Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Feng,Yue Wang,Wei Zhang,Tingting Cai,Xi Tian,Jiaqi Su,Zihao Zhang,Shengfeng Zheng,Shiqi Ye,Bo Dai,Ziliang Wang,Yiping Zhu,Hailiang Zhang,Kun Chang,Dingwei Ye

Journal of diabetes and its complications 34:107444 PubMed31757765

2019

RNA-Seq analysis of the pathogenesis of STZ-induced male diabetic mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ge,Fan Feng,Lanlan Liu,Liang Chen,Peng Lv,Shangshang Ma,Keping Chen,Qin Yao

Frontiers in physiology 9:1051 PubMed30131712

2018

Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ge,Shu Zhang,Liang Chen,Min Tang,Lanlan Liu,Mengna Kang,Lu Gao,Shangshang Ma,Yanhua Yang,Peng Lv,Ming Kong,Qin Yao,Fan Feng,Keping Chen
View all publications
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Product promise

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