AB65596

Anti-CYP7A1 antibody

Name: Anti-CYP7A1 antibody. Rabbit polyclonal (ab65596) | Abcam
Brand: Abcam Limited
SKU: AB65596
Price: 310 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(65 Publications)

Rabbit Polyclonal CYP7A1 antibody. Suitable for WB, ELISA and reacts with Mouse samples. Cited in 65 publications.

View Alternative Names

CYP7, CYP7A1, Cytochrome P450 7A1, 24-hydroxycholesterol 7-alpha-hydroxylase, CYPVII, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase

1 Images

Supplier Data

Western blot - Anti-CYP7A1 antibody (AB65596)

Primary antibody (Green) - ab65596 without (Lanes 1-4) and with (Lanes 5-8) immunising peptide. Loading Control (Red) - ab8245 at 0.1µg/ml, all lanes. Secondary antibody - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed at 1/10000 dilution and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed at 1/10000 Dilution. Blocking Buffer - 3% Milk

All lanes:

Western blot - Anti-CYP7A1 antibody (ab65596) at 1 µg/mL

Lanes 1 and 5:

High Fat Diet Mouse Liver lysate at 20 µg

Lanes 2 and 6:

Mouse Liver lysate at 20 µg

Lanes 3 and 7:

Mouse Thymus lysate at 20 µg

Lanes 4 and 8:

Mouse Colon lysate at 20 µg

Secondary

Lanes 1 - 8:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) at 1/10000 dilution

Lanes 1 - 8:

Goat anti-Mouse IgG H&L (IRDye® 680RD) at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP7A1 also known as cholesterol 7 alpha-hydroxylase plays an important role as an enzyme in the liver. It has a molecular mass of approximately 57 kDa. This enzyme catalyzes the rate-limiting step in the catabolism of cholesterol to bile acids by converting cholesterol to 7 alpha-hydroxycholesterol. The expression of CYP7A1 is mainly in the liver where its activity is important for maintaining cholesterol and bile acid homeostasis.

Biological function summary

Cholesterol 7 alpha-hydroxylase plays an important role in the primary pathway for bile acid biosynthesis. It not only affects the breakdown of cholesterol but also influences lipid metabolism. Although it does not function as part of a larger protein complex CYP7A1 activity is a critical point of regulation within these metabolic processes. Its efficiency can affect plasma cholesterol levels hence impacting overall lipid homeostasis.

Pathways

CYP7A1 is central to the classical pathway of bile acid synthesis. In this pathway it interacts directly with several other proteins including HMG-CoA reductase which is involved in cholesterol synthesis. Additionally CYP7A1 activity influences the regulatory axis of liver X receptors (LXR) which serve to manage cholesterol fatty acids and glucose homeostasis. The balance between these pathways determines cholesterol levels in the body and can modulate metabolic health.

Dysfunction or altered regulation of CYP7A1 contributes significantly to hypercholesterolemia and gallstone disease. Changes in CYP7A1 expression can result in imbalances in cholesterol levels leading to these conditions. The protein's activity is often compared to mitochondrial enzymes related to bile acid synthesis such as CYP27A1 connecting them both in the intricate network of cholesterol regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed : 11013305, PubMed : 12077124, PubMed : 19965590, PubMed : 21813643, PubMed : 2384150). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 11013305, PubMed : 12077124, PubMed : 19965590, PubMed : 21813643, PubMed : 2384150). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed : 12077124, PubMed : 19965590, PubMed : 2384150). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed : 11013305, PubMed : 12077124). Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed : 21813643).

See full target information CYP7A1

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Publications (65)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5015-5033 PubMed40860180

2025

Citrus Pectin Supplementation Alleviated Hepatic Lipid Accumulation through Gut Microbiota Indole Lactic Acid Promoting Hepatic Bile Acid Synthesis and Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Pan,Xinyuan Jin,Qing Li,Yuqing Zhou,Yupeng Zeng,Xin Wang,Yufeng Jin,Yu Chen,Dan Li,Wenhua Ling

Acta pharmaceutica Sinica. B 15:2511-2528 PubMed40487637

2025

A critical role for in negatively impacting metformin response in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Manyun Chen,Yilei Peng,Yuhui Hu,Zhiqiang Kang,Ting Chen,Yulong Zhang,Xiaoping Chen,Qing Li,Zuyi Yuan,Yue Wu,Heng Xu,Gan Zhou,Tao Liu,Honghao Zhou,Chunsu Yuan,Weihua Huang,Wei Zhang

Chinese medicine 20:34 PubMed40069808

2025

Chlorogenic acid attenuates pyrrolizidine alkaloid-induced liver injury through modulation of the SIRT1/FXR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Qiongwen Xue,Aizhen Xiong,Yilin Chen,Xunjiang Wang,Xing Yan,Deqing Ruan,Yumeng Zhang,Zhengtao Wang,Lili Ding,Li Yang

Microbiology spectrum 12:e0083624 PubMed39287458

2024

VSV infection and LPS treatment alter serum bile acid profiles, bile acid biosynthesis, and bile acid receptors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yamei Li,Yan Luo,Chao Wang,Lei Xu,Xinhua Dai,Yunfei An,Lin He,Dongmei Zeng,Yangjuan Bai,Hua Zhang

Nutrients 16: PubMed38542804

2024

Extract Prevents Hepatic Steatosis by Enhancing Bile Acid Synthesis in a High-Fat Diet-Induced Fatty Liver Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Wooyoung Kim,Woon Hee Baek,Sung Ho Yun,Hayoung Lee,Mi Jeong Kim,Sang-Yeop Lee,Gun-Hwa Kim,Seung Il Kim,Hye Gwang Jeong,Edmond Changkyun Park

Molecular nutrition & food research 68:e2300561 PubMed38234006

2024

Gut Microbiota Plays Essential Roles in Soyasaponin's Preventive Bioactivities against Steatohepatitis in the Methionine and Choline Deficient (MCD) Diet-Induced Non-Alcoholic Steatohepatitis (NASH) Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chuhong Su,Jiexian Wang,Huiyu Luo,Junbin Chen,Fengjuan Lin,Jiaqi Mo,Fei Xiong,Longying Zha

EMBO reports 24:e57972 PubMed37962001

2023

MAPL loss dysregulates bile and liver metabolism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Goyon,Aurèle Besse-Patin,Rodolfo Zunino,Olesia Ignatenko,Mai Nguyen,Étienne Coyaud,Jonathan M Lee,Bich N Nguyen,Brian Raught,Heidi M McBride

BMC biotechnology 23:43 PubMed37789318

2023

Engineered FGF19 protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Shi,Tiantian Zhao,Lei Huang,Xiaomin Pan,Tianzhen Wu,Xin Feng,Taoli Chen,Jiamin Wu,Jianlou Niu

Molecular nutrition & food research 67:e2200595 PubMed37148502

2023

Intermittent Fasting Alleviates Non-Alcoholic Steatohepatitis by Regulating Bile Acid Metabolism and Promoting Fecal Bile Acid Excretion in High-Fat and High-Cholesterol Diet Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhuan Lin,Xuan Zhu,Yan Xin,Peiwen Zhang,Yunjun Xiao,Taiping He,Honghui Guo

Frontiers in pharmacology 14:1148737 PubMed37077819

2023

Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis regulation of gut microbiota and bile acid signaling in a mouse model .

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Xin Xin,Qin Feng,Yiyang Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CYP7A1 antibody

Western blot - Anti-CYP7A1 antibody (AB65596)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (65)

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