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AB248849

Anti-CYP7B1 antibody [EPR8395] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CYP7B1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cytochrome P450 7B1, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, Oxysterol 7-alpha-hydroxylase, CYP7B1

2 Images
Western blot - Anti-CYP7B1 antibody [EPR8395] - BSA and Azide free (AB248849)
  • WB

Unknown

Western blot - Anti-CYP7B1 antibody [EPR8395] - BSA and Azide free (AB248849)

This data was developed using ab138497, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CYP7B1 antibody [EPR8395] (<a href='/en-us/products/primary-antibodies/cyp7b1-antibody-epr8395-ab138497'>ab138497</a>) at 1/1000 dilution

Lane 1:

PC3 lysate at 30 µg

Lane 2:

Human fetal liver lysate at 30 µg

Lane 3:

Human fetal kidney lysate at 30 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 55 kDa

false

OI-RD Scanning - Anti-CYP7B1 antibody [EPR8395] - BSA and Azide free (AB248849)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CYP7B1 antibody [EPR8395] - BSA and Azide free (AB248849)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8395

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248849 is the carrier-free version of ab138497.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP7B1 also known as oxysterol 7-alpha-hydroxylase is a member of the cytochrome P450 superfamily of enzymes. This enzyme has a molecular mass of approximately 55 kDa and is mainly expressed in the liver with presence also noted in the kidney brain and other tissues. CYP7B1 catalyzes the hydroxylation of oxysterols and other sterol substrates which is an essential step in cholesterol homeostasis and metabolism. This enzyme functions within the endoplasmic reticulum using NADPH as a cofactor.
Biological function summary

CYP7B1 plays a role in the conversion of cholesterol derivatives into bile acids. It is not typically considered part of a larger protein complex but interacts locally with other enzymes for bile acid synthesis. The enzyme's activity ensures the maintenance of cholesterol balance by converting excess cholesterol into bile acids which are critical for fat digestion and absorption in the intestine. The products of CYP7B1 activity are further metabolized by other enzymes in the bile acid biosynthesis pathways contributing to lipid regulation in the body.

Pathways

CYP7B1 is integral to the alternative bile acid synthesis pathway distinguishing it from the classic pathway. This pathway provides a mechanism to balance cholesterol levels when the primary pathway is insufficient. CYP7B1 also relates functionally to CYP7A1 another enzyme involved in bile acid synthesis although CYP7A1 belongs to the classic pathway. These enzymes ensure that cholesterol conversion is adaptable and responsive to physiological needs maintaining lipid and energy homeostasis.

Mutations in CYP7B1 can lead to hereditary spastic paraplegia type 5 (SPG5) a neurological disorder characterized by progressive lower limb weakness and spasticity. The enzyme’s dysregulation also associates with liver conditions due to cholesterol and bile acid imbalance although the specifics of liver disease involvement remain under investigation. Associated pathways further implicate proteins like ABC transporters that manage lipid transport across membranes in both neurological and hepatic contexts connected to CYP7B1 dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids (PubMed : 10588945, PubMed : 24491228). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 10588945, PubMed : 24491228). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position (PubMed : 10588945, PubMed : 24491228). Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase (PubMed : 24491228). Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids (PubMed : 10588945, PubMed : 9802883). Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response (By similarity). 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning (PubMed : 24491228). Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives (PubMed : 24491228).
See full target information CYP7B1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

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