Rabbit Polyclonal CYP8B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CYP8B1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CYP8B1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNU6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed:10051404). Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity).
Alternative names
CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase
Recommended products
Rabbit Polyclonal CYP8B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CYP8B1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CYP8B1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNU6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CYP8B1 enzyme also known as sterol 12-alpha-hydroxylase plays an important role in bile acid synthesis. It is a member of the cytochrome P450 superfamily with a mass of approximately 58 kDa. This enzyme is mainly expressed in the liver where it catalyzes the conversion of sterols to cholic acid. Through oxidation and hydroxylation reactions CYP8B1 contributes to the balance of bile acid synthesis needed for proper digestion and absorption of lipids.
- Biological function summary
The sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.
- Pathways
CYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.
- Associated diseases and disorders
Disturbances in CYP8B1 activity can lead to metabolic imbalances including gallbladder disease and hypercholesterolemia. Variations in the gene expression or activity of CYP8B1 can affect cholesterol levels potentially resulting in gallstones due to increased cholic acid synthesis. Moreover altered CYP8B1 function can interact with proteins involved in lipid disorders potentially linking to conditions associated with cholesterol accumulation and cardiovascular risk.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CYP8B1 antibody (ab129845)
All lanes: Western blot - Anti-CYP8B1 antibody (ab129845) at 1/500 dilution
Lane 1: 293 cell extract
Lane 2: 293 cell extract with synthesized peptide
Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP8B1 antibody (ab129845)
ab129845, at a dilution of 1/50, staining CYP8B1 in paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry. The picture on the right was treated with synthesized peptide.
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