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AB129845

Anti-CYP8B1 antibody

2

(1 Review)

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(1 Publication)

Rabbit Polyclonal CYP8B1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CYP8B1.

View Alternative Names

CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP8B1 antibody (AB129845)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP8B1 antibody (AB129845)

ab129845, at a dilution of 1/50, staining CYP8B1 in paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry. The picture on the right was treated with synthesized peptide.

Western blot - Anti-CYP8B1 antibody (AB129845)
  • WB

Unknown

Western blot - Anti-CYP8B1 antibody (AB129845)

All lanes:

Western blot - Anti-CYP8B1 antibody (ab129845) at 1/500 dilution

Lane 1:

293 cell extract

Lane 2:

293 cell extract with synthesized peptide

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CYP8B1. The exact immunogen used to generate this antibody is proprietary information.

Q9UNU6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYP8B1 enzyme also known as sterol 12-alpha-hydroxylase plays an important role in bile acid synthesis. It is a member of the cytochrome P450 superfamily with a mass of approximately 58 kDa. This enzyme is mainly expressed in the liver where it catalyzes the conversion of sterols to cholic acid. Through oxidation and hydroxylation reactions CYP8B1 contributes to the balance of bile acid synthesis needed for proper digestion and absorption of lipids.
Biological function summary

The sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.

Pathways

CYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.

Disturbances in CYP8B1 activity can lead to metabolic imbalances including gallbladder disease and hypercholesterolemia. Variations in the gene expression or activity of CYP8B1 can affect cholesterol levels potentially resulting in gallstones due to increased cholic acid synthesis. Moreover altered CYP8B1 function can interact with proteins involved in lipid disorders potentially linking to conditions associated with cholesterol accumulation and cardiovascular risk.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed : 10051404). Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity).
See full target information CYP8B1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 25: PubMed33081083

2020

A Rose Extract Protects the Skin against Stress Mediators: A Potential Role of Olfactory Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Duroux,Anne Mandeau,Gaelle Guiraudie-Capraz,Yannick Quesnel,Estelle Loing
View all publications
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