Rabbit Polyclonal CYP8B1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human CYP8B1.
View Alternative Names
CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase
- WB
Supplier Data
Western blot - Anti-CYP8B1 antibody (AB191910)
All lanes:
Western blot - Anti-CYP8B1 antibody (ab191910)
Lane 1:
HEK293T whole cell lysate
Lane 2:
RAW 264.7 whole cell lysate
Lane 3:
H9C2 whole cell lysate
Predicted band size: 58 kDa
false
- WB
CiteAb
Western blot - Anti-CYP8B1 antibody (AB191910)
CYP8B1 western blot using anti-CYP8B1 antibody ab191910. Publication image and figure legend from Chen, G. & Wu, S., 2020, Gastroenterol Res Pract, PubMed 32382260.
ab191910 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab191910 please see the product overview.
Lithogenic diet induced disorders of cholesterol synthesis and transport in liver. (a–c) The mRNA levels of cholesterol 7α-hydroxylase (CYP7a1) (a), sterol 12α-hydroxylase (CYP8b1) (b), and liver X receptor α (LXRα) (c) in the liver were detected by real-time PCR. (d) Western blot was used to measure the protein levels of CYP7a1, CYP8b1, adenosine triphosphate binding cassette (ABC)G5, ABCG8, and LXRα in the liver of mice that received a lithogenic diet or Baicalin administration. (e–i) Quantitative analysis of CYP7a1 (e), CYP8b1 (f), ABCG5 (g), ABCG8 (h), and LXRα (i) of immunoblot bands in (d). (j) Immunohistochemistry staining was used for detection of LXRα expression in the liver (the scale bar represented 50 μm). n = 6 (*p < 0.05, **p < 0.01, and ***p < 0.001). ns : no significance; BA(L) : 50 mg/kg baicalin; BA(H) : 100 mg/kg baicalin.
false
Reactivity data
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Supplementary information
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Biological function summary
The sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.
Pathways
CYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.
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Publications (22)
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Chinese medicine 20:34 PubMed40069808
2025
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BMC biotechnology 23:43 PubMed37789318
2023
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Nature communications 13:6408 PubMed36302774
2022
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Cell stem cell 29:1366-1381.e9 PubMed36055192
2022
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The Journal of endocrinology 254:137-151 PubMed35608066
2022
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Frontiers in pharmacology 13:875549 PubMed35833020
2022
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International journal of biological sciences 18:3390-3404 PubMed35637968
2022
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Frontiers in medicine 9:818144 PubMed35445045
2022
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Nature communications 13:252 PubMed35017486
2022
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Journal of food science 86:5466-5478 PubMed34730235
2021
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