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AB191910

Anti-CYP8B1 antibody

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(22 Publications)

Rabbit Polyclonal CYP8B1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human CYP8B1.

View Alternative Names

CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase

2 Images
Western blot - Anti-CYP8B1 antibody (AB191910)
  • WB

Supplier Data

Western blot - Anti-CYP8B1 antibody (AB191910)

All lanes:

Western blot - Anti-CYP8B1 antibody (ab191910)

Lane 1:

HEK293T whole cell lysate

Lane 2:

RAW 264.7 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 58 kDa

false

Western blot - Anti-CYP8B1 antibody (AB191910)
  • WB

CiteAb

Western blot - Anti-CYP8B1 antibody (AB191910)

CYP8B1 western blot using anti-CYP8B1 antibody ab191910. Publication image and figure legend from Chen, G. & Wu, S., 2020, Gastroenterol Res Pract, PubMed 32382260.

ab191910 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab191910 please see the product overview.

Lithogenic diet induced disorders of cholesterol synthesis and transport in liver. (a–c) The mRNA levels of cholesterol 7α-hydroxylase (CYP7a1) (a), sterol 12α-hydroxylase (CYP8b1) (b), and liver X receptor α (LXRα) (c) in the liver were detected by real-time PCR. (d) Western blot was used to measure the protein levels of CYP7a1, CYP8b1, adenosine triphosphate binding cassette (ABC)G5, ABCG8, and LXRα in the liver of mice that received a lithogenic diet or Baicalin administration. (e–i) Quantitative analysis of CYP7a1 (e), CYP8b1 (f), ABCG5 (g), ABCG8 (h), and LXRα (i) of immunoblot bands in (d). (j) Immunohistochemistry staining was used for detection of LXRα expression in the liver (the scale bar represented 50 μm). n = 6 (*p < 0.05, **p < 0.01, and ***p < 0.001). ns : no significance; BA(L) : 50 mg/kg baicalin; BA(H) : 100 mg/kg baicalin.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CYP8B1. The exact immunogen used to generate this antibody is proprietary information.

Q9UNU6

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab191910 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYP8B1 enzyme also known as sterol 12-alpha-hydroxylase plays an important role in bile acid synthesis. It is a member of the cytochrome P450 superfamily with a mass of approximately 58 kDa. This enzyme is mainly expressed in the liver where it catalyzes the conversion of sterols to cholic acid. Through oxidation and hydroxylation reactions CYP8B1 contributes to the balance of bile acid synthesis needed for proper digestion and absorption of lipids.
Biological function summary

The sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.

Pathways

CYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.

Disturbances in CYP8B1 activity can lead to metabolic imbalances including gallbladder disease and hypercholesterolemia. Variations in the gene expression or activity of CYP8B1 can affect cholesterol levels potentially resulting in gallstones due to increased cholic acid synthesis. Moreover altered CYP8B1 function can interact with proteins involved in lipid disorders potentially linking to conditions associated with cholesterol accumulation and cardiovascular risk.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed : 10051404). Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity).
See full target information CYP8B1

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 20:34 PubMed40069808

2025

Chlorogenic acid attenuates pyrrolizidine alkaloid-induced liver injury through modulation of the SIRT1/FXR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Qiongwen Xue,Aizhen Xiong,Yilin Chen,Xunjiang Wang,Xing Yan,Deqing Ruan,Yumeng Zhang,Zhengtao Wang,Lili Ding,Li Yang

BMC biotechnology 23:43 PubMed37789318

2023

Engineered FGF19 protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Shi,Tiantian Zhao,Lei Huang,Xiaomin Pan,Tianzhen Wu,Xin Feng,Taoli Chen,Jiamin Wu,Jianlou Niu

Nature communications 13:6408 PubMed36302774

2022

Hepatic thyroid hormone signalling modulates glucose homeostasis through the regulation of GLP-1 production via bile acid-mediated FXR antagonism.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yan,Zhoumin Niu,Chao Sun,Peng Li,Siyi Shen,Shengnan Liu,Yuting Wu,Chuyu Yun,Tingying Jiao,Sheng Jia,Yuying Li,Zhong-Ze Fang,Lin Zhao,Jiqiu Wang,Cen Xie,Changtao Jiang,Yan Li,Xiaoyun Feng,Cheng Hu,Jingjing Jiang,Hao Ying

Cell stem cell 29:1366-1381.e9 PubMed36055192

2022

Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by suppressing intestinal stem cell renewal.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Tingying Jiao,Weiwei Liu,Yuhong Luo,Jue Wang,Xiaozhen Guo,Xiao Tong,Zemin Lin,Chuying Sun,Kanglong Wang,Yifan He,Yuwei Zhang,Hualing Xu,Jiawen Wang,Jianping Zuo,Qiurong Ding,Shijun He,Frank J Gonzalez,Cen Xie

The Journal of endocrinology 254:137-151 PubMed35608066

2022

Hepatic RACK1 deletion disturbs lipid and glucose homeostasis independently of insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Wanying Qin,Ting Zhang,Mingxia Ge,Huimin Zhou,Yuhui Xu,Rongfang Mu,Chaoguang Huang,Daowei Liu,Bangrui Huang,Qian Wang,Qinghua Kong,Qingpeng Kong,Fei Li,Wenyong Xiong

Frontiers in pharmacology 13:875549 PubMed35833020

2022

Theabrownin and Polysaccharide Improve Lipid Metabolism Modulation of Bile Acid and Fatty Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyi Wang,Dan Zheng,Fengjie Huang,Aihua Zhao,Junliang Kuang,Zhenxing Ren,Tianlu Chen,Jing Lei,Jingchao Lin,Xiaoning Wang,Wei Jia,Guoxiang Xie,Xiaojiao Zheng

International journal of biological sciences 18:3390-3404 PubMed35637968

2022

Dynamics of the gut-liver axis in rats with varying fibrosis severity.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Xiang,Zongyi Liu,Huanyu Xiang,Dejuan Xiang,Shuang Xiao,Jing Xiao,Wei Shen,Peng Hu,Hong Ren,Mingli Peng

Frontiers in medicine 9:818144 PubMed35445045

2022

Gypenosides Prevent and Dissolve Cholesterol Gallstones by Modulating the Homeostasis of Cholesterol and Bile Acids.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhuang,Jinnian Cheng,Jie Xia,Min Ning,Shan Wu,Shuang Shen,Yan Shi,Dan Huang,Zhixia Dong,Xinjian Wan

Nature communications 13:252 PubMed35017486

2022

Gut microbiota promotes cholesterol gallstone formation by modulating bile acid composition and biliary cholesterol secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Hu,Wentao Shao,Qian Liu,Ning Liu,Qihan Wang,Jin Xu,Xin Zhang,Zhenkun Weng,Qifan Lu,Long Jiao,Chaobo Chen,Haidong Sun,Zhaoyan Jiang,Xiaoping Zhang,Aihua Gu

Journal of food science 86:5466-5478 PubMed34730235

2021

Policosanol alleviates hepatic lipid accumulation by regulating bile acids metabolism in C57BL6/mice through AMPK-FXR-TGR5 cross-talk.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenya Zhai,Kai-Min Niu,Huiping Liu,Chong Lin,Yue Tu,Yichun Liu,Lichuang Cai,Kexian Ouyang,Jianping Liu
View all publications

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