Rabbit Polyclonal CYP8B1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP8B1 aa 300-450.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYP8B1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNU6
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis (PubMed:10051404). Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase) (By similarity).
Alternative names
CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase
Recommended products
Rabbit Polyclonal CYP8B1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CYP8B1 aa 300-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYP8B1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNU6
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CYP8B1 enzyme also known as sterol 12-alpha-hydroxylase plays an important role in bile acid synthesis. It is a member of the cytochrome P450 superfamily with a mass of approximately 58 kDa. This enzyme is mainly expressed in the liver where it catalyzes the conversion of sterols to cholic acid. Through oxidation and hydroxylation reactions CYP8B1 contributes to the balance of bile acid synthesis needed for proper digestion and absorption of lipids.
- Biological function summary
The sterol 12-alpha-hydroxylase activity of CYP8B1 affects the synthesis of primary bile acids. It does not function as part of a larger protein complex but cooperates with other enzymes in the liver to ensure bile acid balance. The enzyme regulates the ratio between cholic acid and chenodeoxycholic acid influencing cholesterol homeostasis and enterohepatic circulation. Efficient bile acid synthesis by CYP8B1 affects proper lipid breakdown and nutrient absorption in the intestine.
- Pathways
CYP8B1 participates in the bile acid biosynthesis pathway specifically influencing the production of cholic acid. This process impacts the entire lipid metabolism pathway as well as cholesterol metabolism. CYP8B1's activity is complemented by other cytochrome P450 enzymes such as CYP7A1 which also play roles in converting cholesterol to bile acids. These pathways together ensure the regulated production and function of bile acids required for metabolic balance.
- Associated diseases and disorders
Disturbances in CYP8B1 activity can lead to metabolic imbalances including gallbladder disease and hypercholesterolemia. Variations in the gene expression or activity of CYP8B1 can affect cholesterol levels potentially resulting in gallstones due to increased cholic acid synthesis. Moreover altered CYP8B1 function can interact with proteins involved in lipid disorders potentially linking to conditions associated with cholesterol accumulation and cardiovascular risk.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-CYP8B1 antibody (ab236607)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CYP8B1 (green) using ab236607 at 1/100 dilution in ICC/IF. Counter stained with DAPI (blue). The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. The secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP8B1 antibody (ab236607)
Paraffin-embedded human liver tissue stained for CYP8B1 using ab236607 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP8B1 antibody (ab236607)
Paraffin-embedded human liver cancer tissue stained for CYP8B1 using ab236607 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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