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Rabbit Recombinant Monoclonal CYR61/CCN1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.

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Images

Sandwich ELISA - Anti-CYR61 antibody [EPR21133-104] - BSA and Azide free (Capture) (AB244801), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected
Mouse
Expected

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CYR61/CCN1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR21133-104

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab244801 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse CYR61. The recommended pair for sandwich ELISA is:
Capture: ab244801, Human/Mouse CYR61 Capture Antibody (unconjugated)
Detector: Anti-CYR61 antibody [EPR21133-20] - BSA and Azide free (Detector) ab245074, Human/Mouse CYR61 Detector Antibody (unconjugated)
The reference range value is 31.3 - 3000 pg/ml for human and 62.5 - 4000 pg/ml for mouse.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CYR61 also known as CCN1 is a secreted protein involved in cell signaling. It belongs to the CCN family of proteins and weighs approximately 42 kDa. This protein is found in various tissues including the skin lungs and blood vessels. CYR61 is involved in cellular activities such as adhesion migration and proliferation by interacting with cell surface receptors. This active participation in cell signaling makes CYR61 essential to multiple cellular processes.

Biological function summary

CYR61 supports angiogenesis and wound healing by promoting cell survival and adhesion. It functions as a significant component of the extracellular matrix and often participates in complex formations with other CCN proteins. These complexes aid in mediating interactions between cells and their environments. Through these mechanisms CYR61 influences various biological outcomes impacting how tissues grow and repair.

Pathways

CYR61 contributes to both the integrin and TGF-beta signaling pathways. In the integrin pathway it interacts with proteins like integrin alphaVbeta3 to influence cell movement and survival. The TGF-beta pathway involves its regulation of cellular responses to growth factors. By playing important roles in these pathways CYR61 maintains essential cellular functions and intercellular communication impacting diverse physiological processes.

Associated diseases and disorders

CYR61 has links to cancer and fibrosis. Its overexpression can contribute to tumor progression and metastasis often in connection with matrix metalloproteinases during cancer development. In fibrosis CYR61 collaborates with fibroblast growth factor receptors to promote tissue scarring and stiffness. These connections highlight the importance of CYR61 in pathological conditions offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-CYR61 antibody [EPR21133-104] - BSA and Azide free (Capture) (ab244801), expandable thumbnail

    Sandwich ELISA - Anti-CYR61 antibody [EPR21133-104] - BSA and Azide free (Capture) (ab244801)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human CYR61 ELISA Kit ab238267).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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