AB228592

Anti-CYR61/CCN1 antibody

Name: Anti-CYR61/CCN1 antibody (ab228592) | Abcam
Brand: Abcam Limited
SKU: AB228592
Price: 525 USD
Availability: InStock

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(11 Publications)

Rabbit Polyclonal CYR61/CCN1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCN1.

View Alternative Names

CYR61, GIG1, IGFBP10, CCN1, CCN family member 1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, IGF-binding protein 10, IGFBP-10

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody (AB228592)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody (AB228592)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 5 μg/mL Brefeldin A for 24 hours (right panel), stained for CYR61/CCN1 (green) using ab228592 at 1/200 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Paraffin-embedded rat intestine tissue stained for CYR61/CCN1 using ab228592 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Immunohistochemical analysis of paraffin-embedded rat colon tissue stained for secreted CYR61 protein with ab228592 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue stained for secreted CYR61 protein with ab228592 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-CYR61/CCN1 antibody (AB228592)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell extract at 30 µg

Lane 2:

Treated HeLa whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-CYR61/CCN1 antibody (AB228592)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-CYR61/CCN1 antibody (ab228592) at 1/1000 dilution

Lane 1:

786-O whole cell extract at 30 µg

Lane 2:

MCF-7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CCN1. The exact immunogen used to generate this antibody is proprietary information.

O00622

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYR61/CCN1 protein also known simply as CYR61 CCN1 or CYR61 protein is a cellular protein with a molecular weight of approximately 40 kDa. It is a member of the CCN family of regulatory proteins which includes connective tissue growth factors. CYR61 is secreted into the extracellular matrix and has a critical role in cell adhesion and signaling. The protein is expressed in various tissues with higher levels observed in the placenta lungs and kidneys reflecting its role in organ development and repair processes.

Biological function summary

The CYR61/CCN1 protein acts as a signaling molecule that influences cell proliferation migration and adhesion. It interacts with integrins and heparan sulfate proteoglycans on the cell surface facilitating cellular communication that is essential for structural and functional tissue integrity. Additionally CYR61 is a part of multiprotein complexes impacting angiogenesis and inflammation. These activities place CYR61/CCN1 at a pivotal point in wound healing and vascularization processes.

Pathways

The CYR61/CCN1 protein participates in key signaling pathways such as the MAPK/ERK and Wnt pathways. These pathways modulate gene expression related to cell growth and differentiation. CYR61 collaborates with other proteins including VEGF and FGF to regulate cardiovascular development and morphogenesis. The interaction with these pathways highlights the protein’s integral role in cellular homeostasis and response to physiological changes.

CYR61/CCN1 is linked to cancer progression and fibrotic diseases. Elevated levels of this protein are frequently found in breast cancer emphasizing its role in tumor growth and metastasis. It associates with other proteins like TGF-beta during fibrotic responses contributing to the pathology of fibrosis in organs such as the liver and lungs. The involvement in these processes highlights its potential as a therapeutic target in oncology and fibrotic disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

See full target information CCN1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw6926 PubMed40845109

2025

Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yu,Mincheng Yu,Peiyi Xie,Lei Guo,Yufei Zhao,Wenxin Xu,Xian Li,Mengyuan Wu,Zihao Zhang,Zheng Chen,Yongsheng Xiao,Jian Zhou,Jia Fan,Mien-Chie Hung,Yongfeng Xu,Bo Zhang,Qinghai Ye,Hui Li

The Journal of biological chemistry 300:107212 PubMed38522513

2024

The tumor suppressor NF2 modulates TEAD4 stability and activity in Hippo signaling via direct interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengying Wu,Liqiao Hu,Lingli He,Liang Yuan,Lingling Yang,Bin Zhao,Lei Zhang,Xiaojing He

Communications biology 7:130 PubMed38273088

2024

KLF15 transcriptionally activates LINC00689 to inhibit colorectal cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Cao,Jian Li,Gang Zhang,Hao Fang,Yongliang Du,Yan Liang

Frontiers in endocrinology 14:1131993 PubMed37334311

2023

Changes and related factors of blood CCN1 levels in diabetic patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yu Xiang,Shu-Li Chen,Xin-Ran Qin,Sen-Lin Lin,Yi Xu,Li-Na Lu,Hai-Dong Zou

Cancer control : journal of the Moffitt Cancer Center 29:10732748221134398 PubMed36346167

2022

Expression of Shh, Gli1, and Cyr61 in Gastric Cancer Predicts Overall Survival of Patients: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Quan,Zhenming Zhang,Yujie Qin,Xin Gai,Qiling Tian,Yaqiong Guo,Jun Qian,Jiaxi Yao

Cell proliferation 55:e13221 PubMed35355356

2022

FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhicheng Hu,Peng Chen,Linlin Wang,Yu Zhu,Gen Chen,Yunjie Chen,Zhenyu Hu,Lin Mei,Weijing You,Weitao Cong,Litai Jin,Xu Wang,Yang Wang,Xueqiang Guan

Biomedicines 9: PubMed34944635

2021

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuying Liang,Haijing Guan,Jingwen Sun,Yan Qi,Wenjuan Yao

Experimental and therapeutic medicine 22:1437 PubMed34721679

2021

Diagnostic value of using exosome-derived cysteine-rich protein 61 as biomarkers for acute coronary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Yi Li,Wei Zhi,Chen Liu,Weize Fan,Qing Miao,Xinshun Gu

Molecular medicine reports 24: PubMed34505633

2021

Inhibin βA is an independent prognostic factor that promotes invasion via Hippo signaling in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijun Zhang,Shumei Yan,Yan Li,Jiangbo Zhang,Yuan Luo,Pengcheng Li,Yuanzhong Yang,Yong Li,Yuhua Huang,Enhua Wang

Molecular medicine reports 23: PubMed33300071

2020

Dickkopf‑1/cysteine‑rich angiogenic inducer 61 axis mediates palmitic acid‑induced inflammation and apoptosis of vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Rong Gan,Ling Wei,Yan-Zhen Wang,Zong-Ke Kou,Tian-Xiang Liang,Guan-Waner Ding,Yan-Hong Ding,Ding-Xiong Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CYR61/CCN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody (AB228592)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYR61/CCN1 antibody (AB228592)

Western blot - Anti-CYR61/CCN1 antibody (AB228592)

Western blot - Anti-CYR61/CCN1 antibody (AB228592)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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